Figure 4

Representative 1D Western blot for anti-ceruloplasmin expression. Second trimester maternal plasma samples were used (in total, DS [n = 10] and Ctl [n = 10]). Equal amounts of plasma from all samples were separated by gel electrophoresis and blotted with the appropriate dilution of antibody (refer to Materials and Methods). A standard control sample (labelled as 'Standard') was selected and run on every blot to use as a calibration. No significant difference was seen between DS and Ctl levels of ceruloplasmin (CERU; running on 1D gel at 132 kDa).