Table 1 Proteins differentially expressed in the second trimester in the pH range 4.5 - 5.5

1

P00450

Ceruloplasmin

(Seven spots identified for this protein)

CERU_HUMAN

3.69

122205

5.44

173, 437, 431, 460, 331, 107, 110

8, 17, 16, 15, 12, 7, 7

42 - 63

43 - 63

69 - 82

187 - 202

248 - 259

426 - 437

468 - 482

484 - 501

523 - 538

537 - 548

537 - 559

547 - 559

598 - 610

598 - 620

720 - 733

785 - 794

798 - 820

887 - 902

944 - 958

K.KLISVDTEHSNIYLQNGPDR.I

K.LISVDTEHSNIYLQNGPDR.I

K.ALYLQYTDETFR.T

K.DIASGLIGPLIICK.K

K.DNEDFQESNR.M

R.EYTDASFTNR.K

K.GAYPLSIEPIGVR.F

K.NNEGTYYSPNYNPQSR.S

K.EVGPTNADPVCLAK.M

K.M YYSAVDPTK.D

K.M YYSAVDPTKDIFTGLIGPM K.I

K.DIFTGLIGPM K.I

R.M FTTAPDQVDK.E

R.M FTTAPDQVDKEDEDFQESNK.M

R.QSEDSTFYLGER.T

R.QYTDSTFR.V

R.KAEEEHLGILGPQLHADVGDK.V

K.DLYSGLIGPLIVCR.R

K.VNKDDEEFIESNK.M

20.6 (19)

Copper-binding glycoprotein, ferroxidase activity, iron transport

2

Q14624

inter-alpha trypsin inhibitor heavy chain H4

ITIH4_HUMAN

2.11

103357

6.51

74

10

98 - 112

152 - 163

214 - 225

299 - 308

428 - 439

500 - 513

633 - 645

657 - 670

815 - 827

831 - 843

K.AEAQAQYSAAVAK.G

R.RLGVYELLLK.V

R.FKPTLSQQQK.S

K.ILDDLSPR.D

K.LALDNGGLAR.R

R.GPDVLTATVSGK.L

K.IPKPEASFSPR.R

R.M NFRPGVLSSR.L

K.ETLFSVM PGLK.M

K.TGLLLLSDPDK.V

11.5 (10)

Serine-type endopeptidase inhibitor, acute-phase response, hyaluronan metabolic process

3

P0C0L4

complement C4-A precursor (Two spots identified for this protein)

CO4A_HUMAN

2.61

192771

6.66

192, 93

8, 4

756 - 776

916 - 930

979 - 1006

1042 - 1052

1084 - 1100

1291 - 1301

1340 - 1350

1352 - 1366

R.ALEILQEEDLIDEDDIPVR.S

R.GSFEFPVGDAVSK.V

R.VTASDPLDTLGSEGALSPGGVASLLR.L

K.DHAVDLIQK.G

K.VLSLAQEQVGGSPEK.L

R.QGSFQGGFR.S

K.SHALQLNNR.Q

R.GLEEELQFSLGSK.I

6.5 (8)

Activation of the classical pathway of the complement system

4

P01031

complement C5 precursor

CO5_HUMAN

1.59

188305

6.11

154

6

78 - 91

308 - 322

353 - 373

436 - 450

495 - 507

622 - 630

K.FQNSAILTIQPK.Q

K.ELSYYSLEDLNNK.Y

K.LNLVATPLFLKPGIPYPIK.V

K.TDAPDLPEENQAR.E

K.ITHYNYLILSK.G

R.VFQFLEK.S

4.5 (6)

Complement cascade through to formation of membrane attack complex (MAC), inflammatory response

5

P02748

complement component C9 precursor

CO9_HUMAN

2.38

63173

5.43

196

7

65 - 77

144 - 155

145 - 155

231 - 243

472 - 484

496 - 509

533 - 544

R.SIEVFGQFNGK.R

R.DRVVEESELAR.T

R.VVEESELAR.T

K.TSNFNAAISLK.F

K.LSPIYNLVPVK.M

R.AIEDYINEFSVR.K

K.FEGIACEISK.Q

11.8 (7)

Pore-forming subunit of the MAC that plays a key role in the innate and adaptive immune response

6

P01042

kininogen-1 (Two spots identified for this protein)

KNG1_HUMAN

1.72

71957

6.34

37, 187

6, 9

43 - 59

64 - 76

101 - 114

187 - 197

208 - 220

240 - 255

254 - 263

316 - 325

380 - 390

K.YNSQNQSNNQFVLYR.I

K.TVGSDTFYSFK.Y

K.AATGECTATVGK.R

R.QVVAGLNFR.I

K.ENFLFLTPDCK.S

R.IASFSQNCDIYPGK.D

K.DFVQPPTK.I

K.YFIDFVAR.E

K.RPPGFSPFR.S

15.1 (9)

Role in blood coagulation, inhibitor of thiol proteases, inflammatory response, diuresis and natriuresis, smooth muscle contraction, negative regulation of cell adhesion

7

P09871

complement C1s subcomponent precursor (Three spots identified for this protein)

C1S_HUMAN

4.45

76684

4.86

190, 66

8, 5, 6

86 - 106

264 - 281

314 - 332

369 - 384

481 - 497

515 - 524

523 - 535

629 - 645

677 - 688

R.SSNNPHSPIVEEFQVPYNK.L

K.SNALDIIFQTDLTGQK.K

R.DVVQITCLDGFEVVEGR.V

K.VEDPESTLFGSVIR.Y

R.EPTM YVGSTSVQTSR.L

K.LLEVPEGR.T

R.TNFDNDIALVR.L

K.GDSGGAFAVQDPNDK.T

K.TM QENSTPRED.-

18.3 (9)

Serine-type endopeptidase involved in activation of classical pathway of the complement system