Figure 1
From: Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma
Schematic description of the method used in this study for the identification of accessible proteins in Hodgkin lymphoma and reactive lymph nodes. Ex vivo biotinylation of human tissue samples was carried out by incubating fresh tissues in a solution containing reactive ester derivatives of biotin. Proteins from each tissue sample were extracted. Biotinylated proteins were then captured on streptavidin resin, eluted thanks to the disulfide bond between the lysines and the biotin, alkylated and submitted to proteolytic digestion. Biotinylated proteins were sequenced by shotgun mass spectrometry using nLC-ESI MS/MS. Identified proteins differentially expressed in the tissue samples were further validated by immunohistochemical analysis.