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Table 2 Comparisons of total protein concentrations and their percentages of protein loss after different SF sample treatment methods

From: Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis

SF Sample Treatment

Total Protein Concentration in mg/mL (n = number of samples used)

Percentage of Total Protein Loss (%)

Untreated

21.98 ± 1.66 (n = 6)

 

Acetone Precipitation

15.79 ± 0.79 (n = 3)+#

22 ± 8

2-D Clean-Up Kit

19.33 ± 0.98 (n = 3)

14 ± 7

Acetone and 2-D Clean-Up Kit

13.41 ± 0.55 (n = 3)‡*

39 ± 5

  1. Comparison between acetone precipitation method and untreated SF sample: + = p < 0.05.
  2. Comparison between acetone precipitation method and 2-D Clean-Up Kit: # = p < 0.05.
  3. Comparison between combined acetone/2-D Clean-Up Kit treated SF sample, and 2-D Clean-Up Kit treated SF sample: ‡ = p < 0.05.
  4. Comparison between combined acetone/2-D Clean-Up Kit treated SF sample, and untreated SF sample, and SF sample treated with acetone precipitation method: * = p < 0.01.
  5. Values expressed as mean ± standard error of means (SEM).
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