In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Table 3 Changes in regulation of inflammation (ROI) proteins (as compared to KO baseline).

Gel No.	Protein Name	KO 6 hr SP-A	KO 18 hr SP-A	WT
5	Alpha-fetoprotein	↑*	↑*	↑
6	Annexin A2	↓*	↓*	↓*
7	Alpha-fetoprotein	↓	↓	↓
16	Chia protein	↓	↑	↓
17	Chitinase 3-like 3 precursor (Ym1)	↑	↓*	↓*
18	Chitinase-related protein MCRP	↑	↑	↓
24	Eno1 protein (Alpha-enolase)	↑	↓	↓
25	Eukaryotic translation initiation factor 5A	↑	↑	↑
34	Heat shock protein 1, beta (HSP90AB1)	↑	↓	↓
35	Heat shock protein 5 precursor (GRP78)	↑	↓*	↓
36	Heat shock protein 65 (HSP60)	↓	↓*	↓*
37	Heat shock protein 8 (HSC70, HSC71)	↑	↑	↓
38	Heat shock protein 90, beta (Grp94), member 1	↓	↓*	↓*
39	Hematopoietic cell specific Lyn substrate 1	UN	↑	↑
40	Heme-binding protein	↓	↓	↓*
42	High mobility group 1 protein	↑	↓	↑
53	p50b; Leukocyte-specific protein 1 (LSP1)	↓	↓	↓
72	Tyrosine 3/tryptophan 5-monooxygenase activation protein, ε	↑	↓	↓
73	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β	↑	↓	↓
76	Vimentin	↑	↓	↓*
	Total changes	7↓, 12↑, 1UN	14↓, 6↑	16↓, 4↑
	Total significant changes	1↓, 1↑	5↓, 1↑	6↓*

Comparison of mean normalized volumes (see Additional File 4) for proteins from KO mice to KO 6 hr SP-A, KO 18 hr SP-A, and wild-type mice. Increased (↑), decreased (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*).

ISSN: 1477-5956