The new platinum-based anticancer agent LA-12 induces retinol binding protein 4 in vivo

Table 1 Rat RBP4 protein identification data

Plasma retinol-binding protein precursor (RBP4)
UniProt Accession: P04916 (RET4_RAT)
Protein Score		472
Protein Score C.I. %		100
Total Ion Score		394
Total Ion Score C.I. %		100
Sequence Coverage %		55
E-value		3.3 × 10^-42
Obsrv. Mass	Calc. Mass	± Da	Start Seq.	End Seq.	Miss Cleav.	Ion Score	C.I. %	E-value	Peptide Sequence+Modification
1155.6251	1154.6124	0.0054	38	47	0	---	---	---	R.FSGLWYAIAK.K
1226.6394	1225.6243	0.0078	108	117	0	---	---	---	K.YWGVASFLQR.G
1343.6510	1342.6299	0.0138	172	181	1	---	---	---	R.QRQEELCLER.Q + Pyro-glu (N-term Q)
1360.6714	1359.6565	0.0077	172	181	1	27	39	0.85	R.QRQEELCLER.Q
2079.9431	2078.9367	-0.0009	140	157	0	148	100	2.9e-13	R.LQNLDGTCADSYSFVFSR.D
2129.0942	2128.1138	-0.0269	1	20	0	---	---	---	-.MEWVWALVLLAALGGGSAER.D
2279.1006	2278.1004	-0.0071	49	68	0	---	---	---	K.DPEGLFLQDNIIAEFSVDEK.G
2407.1960	2406.1954	-0.0067	48	68	1	132	100	2.1e-11	K.KDPEGLFLQDNIIAEFSVDEK.G
2705.1455	2704.1500	-0.0118	118	139	0	87	100	8.6e-8	R.GNDDHWIIDTDYDTFALQYSCR.L

Table includes protein name, UniProt accession number and entry name, Protein Score, Protein Score C.I. %, Total Ion Score, Total Ion Score C.I. %, Sequence Coverage %, E-value; list of peptides matched to the identified protein with their observed and calculated m/z values, error of the measurement (Da), start and end position in protein sequence, number of missed cleavages, Ion Score, C.I. % for peptides confirmed by MS/MS, their E-value, sequences of identified peptides and note about their modification. The data correspond to gel piece excised from the gel as shown in Figure 2, no other protein was identified with significant score in tryptic digest of this gel band.

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