Table 2 Proteins differentially expressed in hearts of ACF rats.

Peptides (95% confidence)

Seq. Cov.

Accession

Protein name

Protein Fold-change (iTRAQ ratio)

mRNA fold-change

53

54

gi|259435950

Long-chain-fatty-acid-CoA ligase 1

0.23

NA

17

25

gi|59797483

Carnitine O-acetyltransferase

0.24

0.52

124

66.1

gi|189083744

Sarcomeric mitochondrial creatine kinase

0.24

1.05

42

63

gi|54035288

Enolase 3, beta

0.26

0.23

26

52

gi|57333

3-2 trans-enoyl-CoA isomerase

0.27

0.54

40

49

gi|60688124

Trifunctional enzyme subunit alpha, mitochondrial (HADHA)

0.3

0.54

24

35

gi|31077132

Histidine rich calcium binding protein

0.31

0.61

9

37

gi|1906812

Inducible carbonyl reductase

0.32

0.45

49

65

gi|56541110

Acyl-Coenzyme A dehydrogenase, very long chain

0.33

0.59

28

45

gi|510110

Trifunctional enzyme subunit beta, mitochondrial (HADHB)

0.33

0.56

4

17

gi|66910891

Glutamic-pyruvate transaminase (alanine aminotransferase)

0.34

0.38

113

53

gi|57303

Sarcoplasmic reticulum 2+-Ca-ATPase (SERCA2)

0.35

1.0

40

56.8

gi|149042663

Sarcalumenin

0.36

0.91

20

41.1

gi|77993368

Acyl-CoA synthetase family member 2 precursor

0.39

NA

120

74.3

gi|6978661

Muscle creatine kinase

0.4

0.69

195

75.8

gi|83300587

ATP synthase subunit alpha, mitochondrial;

0.4

0.71

120

71

gi|62079055

Isocitrate dehydrogenase 2 (NADP+)

0.41

0.62

30

50

gi|7387725

Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase

0.43

0.37

18

47.5

gi|51260066

Propionyl coenzyme A carboxylase, beta polypeptide

0.43

0.84

19

39

gi|6166586

Acyl-coenzyme A thioesterase 2

0.44

0.54

24

42.6

gi|149050263

Propionyl-CoA carboxylase alpha chain

0.44

0.91

35

40.7

gi|6978543

Na+/K+ -ATPase alpha 1 subunit precursor

0.45

1.1

34

64

gi|56929

Pyruvate kinase M1/M2

0.46

0.6

16

37

gi|62825891

Phosphofructokinase, muscle

0.46

0.5

42

68.8

gi|57527204

Electron-transfer-flavoprotein, alpha polypeptide

0.46

0.69

10

30

gi|149062241

LRP16 protein

0.47

0.38

35

47.9

gi|92090591

Glutamate dehydrogenase 1

0.47

0.84

13

43

gi|6981396

Protein kinase, cAMP dependent regulatory, type I, alpha

0.47

1.0

68

37

gi|61557127

Nicotinamide nucleotide transhydrogenase

0.48

0.67

111

69.1

gi|6978431

Long-chain acyl-CoA dehydrogenase precursor

0.49

0.84

31

49

gi|48734846

Acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain

0.53

0.58

64

44.5

gi|81883712

2-oxoglutarate dehydrogenase E1 component

0.53

0.69

48

67

gi|149027156

Acetyl-Coenzyme A acyltransferase 2

0.54

0.61

45

25.9

gi|189181710

Ryanodine receptor 2, cardiac

0.58

0.79

30

37

gi|81871846

Leucine-rich PPR motif-containing protein, mitochondrial

0.61

0.66

33

45

gi|6978705

Carnitine O-palmitoyltransferase precursor

0.61

0.58

Proteins upregulated in ACF

Peptides (95% confidence)

Seq. Cov.

Accession

Protein name

Protein Fold-change (iTRAQ ratio)

mRNA fold-change

44

55

gi|48425083

Monoamine Oxidase A

4.06

1.93

10

18

gi|55249666

Cadherin 13

3.40

2.15

19

34

gi|5326787

Transglutaminase 2

3.07

1.93

24

61

gi|94400790

Heat shock protein 1 (HSP27)

3.05

1.41

23

72.2

gi|438878

tropomyosin

3.04

1.32

10

42

gi|6978501

Annexin A1

3.00

2.23

35

69.6

gi|535069

Muscle LIM protein [Rattus norvegicus]

2.97

1.31

22

50

gi|6981324

Prolyl 4-hydroxylase, beta polypeptide

2.91

1.27

59

73.3

gi|56388799

Brain creatine kinase (Ckb protein)

2.88

1.31

16

30

gi|149048530

Ceruloplasmin, isoform CRA_a

2.80

2.02

34

62.3

gi|744592

Alpha-B crystallin

2.61

1.05

35

68

gi|157830232

Annexin V

2.58

1.71

20

27.3

gi|462569

Microtubule-associated protein 1A

2.58

1.30

10

26.9

gi|158706096

Pre-B-cell leukemia transcription factor-interacting protein 1

2.45

1.23

8

34.4

gi|68837285

D-beta-hydroxybutyrate dehydrogenase, mitochondrial;

2.44

1.02

10

28

gi|974168

Aldehyde dehydrogenase 1A1 (retinal dehydrogenase 1)

2.43

1.84

11

28

gi|7533042

Guanine deaminase

2.41

2.02

8

28.9

gi|57241

Sulfated glycoprotein 2 (clusterin)

2.39

1.34

24

38.6

gi|6981022

Hexokinase 1

2.23

NA

59

64.4

gi|109468300

Alpha-enolase (Enolase 1)

2.23

1.00

94

50.7

gi|149063941

Beta myosin heavy chain myo7

2.22

1.02

14

35.3

gi|53237076

EH-domain containing 4

2.22

1.08

22

50

gi|9845234

Annexin A2

2.21

2.17

11

25

gi|149018456

Microtubule-associated protein 4

2.18

1.24

6

26

gi|158186676

Calumenin isoform a

2.17

0.86

39

42.6

gi|54673763

Heat shock protein 90, alpha (cytosolic), class A member 1

2.14

1.27

13

60.8

gi|1051270

14-3-3 zeta isoform

1.99

1.18

11

33.2

gi|55855

Calreticulin

1.90

1.18

41

26.9

gi|62646949

Filamin-C (Gamma-filamin) (Filamin-2)

1.87

1.21

18

39.1

gi|157819677

Sarcolemma associated protein

1.81

1.02