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Figure 1 | Proteome Science

Figure 1

From: Proteomic analysis of secretagogue-stimulated neutrophils implicates a role for actin and actin-interacting proteins in Rac2-mediated granule exocytosis

Figure 1

Kinetic analysis of neutrophil exocytosis. Neutrophils were isolated from bone marrow of wild-type and Rac2-/- mice. 1 × 106 cells were incubated with 5 μM CB for 5 min followed by 5 μM fMLF. At the indicated times samples were taken and cell-free supernatants were analyzed for MPO, an enzyme contained in primary granules. Exocytosis was measure as the % MPO release, calculated as a ratio of total cellular MPO. A. Shown is a representative result from a typical degranulation assays. B. Pre-incubation of BMN with 10 μM cycloheximide for 1 hr at 37°C prior to CB/fMLF stimulation does not affect degranulation (n = 3).

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Proteome Science

ISSN: 1477-5956