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Table 2 Proteins identified by mass spectrometry and their differences in spot abundance compared between Rac2-/ - unstimulated and stimulated BMN.

From: Proteomic analysis of secretagogue-stimulated neutrophils implicates a role for actin and actin-interacting proteins in Rac2-mediated granule exocytosis

Identity

Master

Number

NIH protein database accession number

Rac2-/- Unstimulated (abundance)

Rac2-/- Stimulated (abundance)

Ratio

p-value

(t test)

Coronin

691

NP_034028

0.0655

0.0990

1.51

0.13

HSP60

382

NP_034607

0.0572

0.0652

1.14

0.14

GAPDH

425

AAU89484

0.0696

0.0731

1.05

0.56

Granule protein 1

965

BAB26414

0.244

0.151

-1.62

3.1 × 10-2

β-actin

637

ABL01512

0.0729

0.0517

-1.41

1.5 × 10-2

F-actin capping protein (CapZ-β)

999

NP_033928

0.161

0.0951

-1.69

5.9 × 10-3

Granule protein 2

969

NP_032720

0.158

0.0891

-1.79

2.2 × 10-3

Chitinase

1018

NP_075675

4.39

2.23

-1.97

4.9 × 10-4

Granule protein 3

970

EDL09016

0.0539

0.0258

-2.09

1.6 × 10-2

β-actin

985

ABL01512

0.342

0.151

-2.25

4.4 × 10-3

  1. HSP60, heat shock protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NIH, National Institutes of Health.