Proteomic analysis of secretagogue-stimulated neutrophils implicates a role for actin and actin-interacting proteins in Rac2-mediated granule exocytosis

Table 3 Proteins identified by mass spectrometry and their differences in spot abundance compared between wild-type stimulated and Rac2^-/ ^- stimulated BMN.

Identity	Master Number	NIH protein database accession number	Wild-type Stimulated (abundance)	Rac2^-/- Stimulated (abundance)	Ratio	p-value (t test)
Granule protein 3	970	EDL09016	0.0108	0.0258	2.39	4.1 × 10^-4
Granule protein 2	969	NP_032720	0.0598	0.0891	1.49	0.18
F-actin capping protein (CapZ-β)	999	NP_033928	0.0478	0.0951	1.99	6.1 × 10^-2
β-actin	637	ABL01512	0.0306	0.0517	1.69	1.1 × 10^-2
Granule protein 1	965	BAB26414	0.0728	0.151	2.07	2.6 × 10^-3
β-actin	985	ABL01512	0.0898	0.152	1.69	7.3 × 10^-2
Chitinase	1018	NP_075675	0.803	2.23	2.77	9.0 × 10^-5
GAPDH	425	AAU89484	0.128	0.0731	-1.75	7.9 × 10^-5
HSP60	382	NP_034607	0.105	0.0652	-1.61	6.2 × 10^-4
Coronin	691	NP_034028	0.192	0.0990	-1.94	4.0 × 10^-3

HSP60, heat shock protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NIH, National Institutes of Health.

ISSN: 1477-5956