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Figure 1 | Proteome Science

Figure 1

From: Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

Figure 1

Silver nitrate stained 2-DE gel. (A) Proteins (160 μg) were separated in the first dimension using non-linear pH 3-10 gradient IPG strips (17 cm, Bio-Rad), followed by second dimension on 12.5% SDS-PAGE. Consistently regulated spots were excised from silver stained gel after densitometric analysis for identification by Q-TOF MS/MS analysis. Spots marked on the gel showed differentially regulated proteins. Note: "P" refers to phospho protein spots also shown in figure 2. (B) Two representative differentially regulated 2-DE spots (MOBKL1A, spot 8; SNAPA, spot 10) in non-heated FCS with low LPS (NHL) and in heated FCS with normal LPS concentration (HE) respectively. The spot IDs correspond to the listing in Table 1. The error bars represent mean ± SD (*= p < 0.05, **= p < 0.005) of six independent experiments.

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Proteome Science

ISSN: 1477-5956