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Table 1 Identified proteins whose expression levels changed between Arabidopsis thaliana wild-type and pin 2 root tips subjected to horizontal clinorotation compared to those under stationary control using two-dimensional electrophoresis and liquid chromatography- ion trap mass spectrometry

From: A proteomic approach to analyzing responses of Arabidopsis thaliana root cells to different gravitational conditions using an agravitropic mutant, pin2 and its wild type

Spot

Noa)

Obs.b)

Mr/pI

Identity of the proteins

No.

Matd)

% Sq

Cove)

Theo.c)

Mr/pI

Acces.

no.f)

H/Sg)

       

WT

pin2

  

Group I

      

9

65/5.66

TCP-1 chaperonin-like protein

14/10

23.52

59/5.97

CAC01806.1

1.5

2.2

22

54/6.00

ATP synthase beta chain 2, mitochondrial

47/21

53.42

60/6.18

sp[P83484]

1.7

1.7

23

44/6.12

Mevalonate diphosphate decarboxylase

3/2

8.98

46/6.33

CAA76803.1

1.8

2.1

30

45/5.72

Isocitrate dehydrogenase

60/20

50.24

46/6.13

CAD24782.1

2.3

2.4

19

54/5.76

Aldehyde dehydrogenase (NAD+)-like protein

4/4

12.08

58/7.11

CAB41139.1

2

2.5

24

44/6.42

Glutathione-dependent formaldehyde dehydrogenase class III ADH

24/9

31.93

41/6.51

CAA57973.1

1.9

2.5

16

57/6.08

Putative inosine-5'-monophosphate dehydrogenase

9/6

21.07

54/6.34

sp[Q9SA34]

2.6

2.1

47

25/5.73

Glutathione S-transferase 6

3/2

17.67

24/6.09

sp[Q96266]

1.9

3.3

  

GroupII

      

15

60/5.77

Putative malate oxidoreductase

21/13

33.00

66/6.85

CAB80866.1

2.4

NC

21

54/6.00

ATP synthase alpha chain, mitochondrial

89/20

42.60

55/6.23

sp[P92549]

2.0

NC

20

55/5.79

Enolase(2-phosphoglycerate dehydratase)

24/13

52.03

48/5.54

sp[P25696]

1.8

NC

25

41/6.66

Glyceraldehyde-3-phosphate dehydrogenase

44/14

62.13

37/6.62

sp[P25858]

2.2

NC

42

29/5.01

Cytosolic triose-phosphate isomerase

23/10

43.70

27/5.39

CAB75902.1

2.1

NC

46

27/5.18

Protein F18O14.33 [imported]

4/3

8.86

50/6.81

D86328

1.8

NC

53

18/6.58

Hypothetical protein At2g26210 [imported]

6/2

35.56

9.2/6.5

G84657

1.9

NC

26

41/6.79

Peroxidase

65/23

41.76

39/6.21

CAA66959.1

0.3

NC

38

32/6.64

Adenylate kinase 1

3/2

13.01

27/6.91

sp[O82514]

p

NC

39

31/6.62

Adenylate kinase 1

3/2

14.63

27/6.91

sp[O82514]

p

NC

40

31/6.63

Adenylate kinase 1

9/4

27.24

27/6.91

sp[O82514]

p

NC

  

Group III

      

12

58/4.56

Probable ubiquitin-like protein [imported]

2/1

2.90

58/4.81

C84549

NC

2.0

74

43/5.83

Alpha-galactosidase-like protin

3/3

10.49

46/6.19

CAC08338.1

NC

2.1

82

31/5.78

Hydroxyacylglutathione hydrolase cytoplasmic (glyoxalase II)

5/3

24.81

29/5.93

sp[O24496]

NC

1.7

36

36/5.48

Annexin 2

2/1

46.34

4.7/4.8

BAD94993.1

NC

2.4

  1. a) Index in the reference gel. b) Observed Mr and pI which were estimated by the electrophoresis mobilities. c) Expected Mr and pI which were calculated on the complete sequences. d) Peptides had been identified, the number before solidus represent amount of the peptides had been identified containing overlaps and the number behind solidus represent amount of the peptides excluding overlaps. e) Percentage that the mass of the peptides we identified account for the percentage of total mass of the protein. f) Accession number in NCBI, SWISS-Port, or PIR. g) H/S, ratio of amount of identified protein of the horizontal clinorotation treated samples and the stationary control. NC, no change; p, position of identified protein in gel changed after treatment; H, horizontal clinorotation; S, 1 g stationary control. Group I, proteins showed altered expression in both WT and pin2 under the horizontal clinorotation in comparison with stationary controls. Group II, expression of proteins was modified in only WT but apparently unchanged in pin2 under the horizontal clinorotation. Group III, expression of proteins did not changed in WT roots, but significantly up-regulated in pin2 roots subjected to the horizontal clinorotated treatment.
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