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Table 2 Identified proteins whose expression levels changed between Arabidopsis thaliana wild-type and pin 2 root tips exposed to hyper-gravity force (7 g) compared to 1 g control using two-dimensional electrophoresis and liquid chromatography-ion trap mass spectrometry

From: A proteomic approach to analyzing responses of Arabidopsis thaliana root cells to different gravitational conditions using an agravitropic mutant, pin2 and its wild type

Spot

Noa)

Exp.b)

Mr/pI

Identity of the proteins

No.

Matd)

% Sq

Cove)

Theo.c)

Mr/pI

Acces.

no.f)

G/S g)

       

WT

pin 2

  

Group I

      

9

65/5.66

TCP-1 chaperonin-like protein

14/10

23.52

59/5.97

CAC01806.1

2.6

2.2

22

54/6.00

ATP synthase beta chain 2, mitochondrial

47/21

53.42

60/6.18

sp[P83484]

1.5

1.9

23

44/6.12

Mevalonate diphosphate decarboxylase

3/2

8.98

46/6.33

CAA76803.1

1.6

2.0

30

45/5.72

Isocitrate dehydrogenase

60/20

50.24

46/6.33

CAD24782.1

1.5

1.9

19

54/5.76

Aldehyde dehydrogenase (NAD+)-like protein

4/4

12.08

58/7.11

CAB41139.1

2.5

0.5

36

36/5.48

Annexin 2

2/1

46.34

4.7/4.8

BAD94993.1

1.8

3.1

21

54/5.84

Mitochondrial ATP synthase alpha chain

89/20

42.60

55/6.23

sp[P92549]

0.4

0.5

2

73/4.95

Heat shock cognate 70 kDa protein 1

48/21

42.24

71/5.03

sp[P22953]

8.0

15

56

73/5.77

Heat shock cognate 70 kDa protein 1

19/11

24.88

71/5.03

sp[P22953]

13.0

5.7

3

74/5.02

Heat shock cognate 70 kDa protein 3

54/23

42.99

71/4.97

sp[O65719]

2.8

15.0

57

68/5.76

Stress-induced protein sti1-like protein

54/24

46.77

64/6.00

CAB78283.1

4.7

1.6

5

78/5.61

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor

10/6

13.10

82/6.24

sp [Q 9FGI6 ]

6.4

1.8

38

32/6.64

Adenylate kinase 1

3/2

13.01

27/6.91

sp[O82514]

p

p

39

31/6.62

Adenylate kinase 1

3/2

14.63

27/6.91

sp[O82514]

p

p

40

31/6.63

Adenylate kinase 1

9/4

27.24

27/6.91

sp[O82514]

p

p

  

Group II

      

15

60/5.77

Putative malate oxidoreductase

21/13

33.00

66/6.85

CAB80866.1

2.3

NC

24

44/6.42

Glutathione-dependent formaldehyde dehydrogenase class III ADH

24/9

31.90

41/6.51

CAA57973.1

0.3

NC

  

Group III

      

74

43/5.83

Alpha-galactosidase-like protein

3/3

10.49

46/6.19

CAC08338.1

NC

2.1

82

31/5.78

Hydroxyacylglutathione hydrolase cytoplasmic (glyoxalase II)

5/3

24.81

29/5.93

sp[O24496]

NC

2.0

  1. a)-f) is as the description in the table 1. g) G/S, ratio of amount of identified protein of the hyper-gravity treated samples and its stationary control. NC, no change; p, position of identified protein in gel changed after treatment; G, hyper-gravity treatment; S, 1 g stationary control. Group I, proteins showed altered expression in both WT and pin2 under the hypergravity condition in comparison with the stationary controls. Group II, expression of proteins was modified in only WT but apparently unchanged in pin2 under the hypergravity. Group III, expression of proteins did not changed in WT roots, but significantly increased in pin2 roots subjected to the hypergravity treatment.
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Proteome Science

ISSN: 1477-5956