Figure 1
From: Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Experimental workflow - The process begins with (1.) the distribution of samples into microtiter plates according to a defined layout, dilution and heat treatment. (2.) The protein content of the samples is then label with biotin and (3.) the samples are then prepared for the assay and heat-treated again. Alongside this, (4.) the antibodies are coupled onto beads with distinct color-codes and an array in suspension is created and (5.) beads and samples are combined and incubated. (6.) Proteins that have not been captures by the antibodies are removed and (7.) fluorescent streptavidin is added to bind to the target proteins via their biotin modification. (8.) The beads are then measured and the co-occurrence of beads, which are identified via a green laser, and the emitted reporter fluorescence, excited by a red laser, allowing determining interaction dependent intensity values in multiplex.