Figure 4

Western blot of αB-crystallin and amyloid precursor protein expression in s-IBM. Through semi-quantitative analysis using gray-scale quantification, steady-state expression levels of αB-crystallin and APP in s-IBM were found to be significantly increased relative to controls (P < 0.05). β-actin was used as an internal control, and protein expression was normalized by taking the target protein/β-actin.