Two dimensional gel phosphoproteome of peripheral blood mononuclear cells: comparison between two enrichment methods

Table 1 Phosphorylated PBMCs’ proteins differentially expressed between the two phosphoprotein pattern (LaCl ₃ versus affinity micro-column)

Protein name	Accession number	Molecular weight (Da)	Mascot score	Seq. Cov (%)	Phosphorylation sites	P value^a
Protein name	Accession number	Molecular weight (Da)	Mascot score	Seq. Cov (%)	Phosphorylation sites	LaCl3vscolumn (fold change)
Filamin-A (fragment)	P21333	280739	74	16	17^b + 147^c	0.02
Filamin-A (fragment)	P21333	280739	74	16	17^b + 147^c	(4.0)
Coronin-1A	P31146	51678	171	40	2^b + 9^c	0.02
Coronin-1A	P31146	51678	171	40	2^b + 9^c	(15.0)
Pyruvate kinase isozymes M1/M2 (fragment)	P14618	57937	92	21	5^b + 28^c	0.02
Pyruvate kinase isozymes M1/M2 (fragment)	P14618	57937	92	21	5^b + 28^c	(3950)
Ficolin-1	O00602	35078	78	17	2^b	0.02
Ficolin-1	O00602	35078	78	17	2^b	(1.92)

^a)Mann Whitney U-test. ^b)Phosphorylation sites described in UniProtKB (http://www.uniprot.org) and in ^c)PhosphoSitePlus (http://www.Phosphosite.org).

ISSN: 1477-5956