Figure 1

MARS affinity column depleted high abundance proteins. A. Flow diagram for depletion of seven high-abundance proteins from human plasma. B. Equal amounts (35 μg) of the total, bound and depleted plasma were loaded in the indicated lane, separated on a gradient SDS-PAGE gel (7.5-15%), and stained with coomassie blue. Positions 1, 3, 4 and 6 indicate enrichment after depletion, position 2 shows the appearance of comigrated bands in depleted fraction with high abundance proteins, whereas 5 and 7 positions represent decrease or removal after depletion. C. Total and depleted plasma (250 μg) were separated on 3-10 pI range IPG strip (17 cm) with 6-18% gradient gel and stained with coomassie blue. "[" marked portion indicates depletion of proteins while alphabetically marked positions (a, b) indicate the appearance of some co-migrated spots after depletion.