Figure 2
From: Comparative proteomics and glycoproteomics of plasma proteins in Indian visceral leishmaniasis
Comparative 2DE and 2D-DIGE analysis in the acidic pI range. A. Equal amount (50 μg) of depleted endemic control, VL and non-endemic control plasma samples were separated in 4-7 pI range IPG strip (17 cm) followed by SDS-PAGE (6-18%) and silver stained. Differentially expressed spots were circled with numbers after visual comparison between VL and endemic/non-endemic controls. B. C. Equal amount (50 μg) of depleted VL and endemic/non-endemic control plasma proteins labeled with CyDyes and separated. Images of VL and control were taken at different excitation/emission wavelengths and merged with ImageQuant Tool. Differentially expressed spots were marked by numbers following DIA analysis. D. Zoomed images and the analytic three dimensional expression profile of marked differently expressed spots 1-7.