Figure 3
From: Comparative proteomics and glycoproteomics of plasma proteins in Indian visceral leishmaniasis
VL-associated alterations in narrow acidic pI range. A. Depleted VL and endemic/non-endemic control plasma proteins (50 μg) were focused using 4.7-5.9 pI range IPG strip (17 cm) and followed by SDS-PAGE (6-18%). Gels were silver stained and differentially expressed spots (1-12) were marked after visual comparison. B. C. Equal amounts (50 μg) of VL and endemic/non-endemic control were labeled by CyDyes with internal standard and separated as A. Fluorescent images were taken at different excitation/emission wavelengths and marked by numbers after DIA analysis. D. The magnified images and three dimensional expression profiles of the differentially expressed spots compared to controls were reported.