Figure 5
From: Comparative proteomics and glycoproteomics of plasma proteins in Indian visceral leishmaniasis
Comparative glycoproteomics using 2DE and 2D-DIGE analysis. A. Equal amounts (50 μg from each) from VL and controls (endemic/non-endemic) were separated using 4-7 linear pI range IPG strip (17 cm) and SDS-PAGE (6-18%). The gels were silver stained and differential expressions of VL glycoproteins compared to both endemic and non-endemic control were marked with an arrow and number after visual comparison. B. C. Purified glycoproteins (50 μg) from VL and endemic/non-endemic controls were labeled with CyDyes with corresponding internal standard and separated as A. Gels were scanned in different excitation/emission wavelengths and Cy3 and Cy5 images were merged using ImageQuant tool. Differently expressed spots were marked by numbers. D. Zoomed images of differentially expressed spots and 3D expression profile of the same after DIA analysis.