Figure 4

Top10 gene ontology classification to cellular compartment. The percentages of the numbers of proteins (left) as well as of the sum of the peptides measured (right) were given. a) The crude EV preparations resulted in an increased number of cytoplasmic and membranous originating proteins and a reduction of extracellular proteins, compared to the secretome and lysate of Panc1. The "enriched crude" was named the in silico enriched fraction, representing the proteins which were more frequently found in crude EV samples than in the secretomes. (The analysis for HPDE samples were given in Additional file 1: Figure S5). b) By the affinity purification procedure, the number of membranous proteins and cytoplasm originating proteins was drastically expanded and the extracellular classified proteins were reduced.