Table 6 The difference of SDS-PAGE and 2-DE map of wild rice species and identification of the corresponding protein spots among three wild rice species
From: Proteomic analysis of seed storage proteins in wild rice species of the Oryza genus
Differential protein region | SDS-PAGE | 2-DE map | *2-DE map identification by Xie et al.[[12]] | LC/ESI-MS/MS identification of wild rice species |
|---|---|---|---|---|
I, Glutelin acidic subunit | Expression of protein from 35Â kDa to 34Â kDa in this area of O. meyeriana was significantly higher than that of other materials. | The expression of protein spot 14, spot 15 and spot 16 with molecular weight 34 kDa and pI 8.0 to 9.5 obviously increased in O. meyeriana. | Corresponding to spots 15 and 16 in the corresponding region which were identified as the 56Â kDa putative glutelin type-B 2 precursor | No |
α-1 subunit of glutelin acidic subunit was absent, while a new protein band of about 40 kDa appeared in O. meyeriana. | Specific protein spot 23 (34 kDa, pI 5.95), spot 24 (34 kDa, pI 5.72) and spot 25 (34 kDa, pI 5.54) were observed in O. officinalis; Protein spot 7 (35 kDa, pI 7.22), spot 13 (34 kDa, pI 7.58) and spot 14 (34 kDa, pI 8.11) were absent in O. officinalis;. | Corresponding to spot 14 in the corresponding region which was identified as the 56 kDa putative glutelin type-B 2 precursor. | Protein spot 14: hypothetical protein | |
| Â | Two unique protein spots 21 (pI 6.68,34Â kDa) and 22 (pI 6.83,34Â kDa) were detected in O. rufipogon. | No corresponding spot in the corresponding area. | Protein spot 23: 56 kD glutelin type-A 2 precursor | |
II, Glutelin precursor | The expression of 57Â kDa glutelin precursor increased in O. rufipogon. | The expression of protein in this region with 57Â kDa, and pI 9.0-10.0 increased in the three wild rice species | Corresponding to spot 5 in the corresponding region which was identified as 56Â kDa glutelin type I precursor. | No |
IV, Water-soluble protein | The expression contents of 62 kDa protein in wild rice species were higher than two cultivated rice materials. | Four consecutive protein spots 1, 2, 3 and 4 with 62Â kDa and pI from 4.5 to 7.5 were elevated in water-soluble protein area of the three wild rice species. | Corresponding to spots 1, 2, 3 and 4 in the corresponding region which were identified as glycogen (starch) synthase. | No |
| Â | Two unique protein groups were present in O. meyeriana. | No corresponding spot in the corresponding area. | No | |
V, Globulin | The 26 kDa protein band was absent, and a new 28 kDa protein band appeared in O. meyeriana. | Protein spots 17 (pI 5.44, 24 kDa) and 18 (pI 5.65, 24 kDa) were absent in O. officinalis, meanwhile a protein spot 39 with 24 kDa and pI 6.27 was present. | Corresponding to spots 17 and 18 in the corresponding region which were identified as 21 kDa α globulin, and the experimental protein molecular weight is 26 kDa. | No |
The 26 kDa protein band in O. officinalis decreased remarkably. | The expression of protein spot 18 notably higher than the other material in O. meyeriana. |