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Table 3 Spiking recovery

From: Mass Spectrometric Immunoassay for the qualitative and quantitative analysis of the cytokine Macrophage Migration Inhibitory Factor (MIF)

Sample

MIF added

Observed

Expected

Recovery

 

ng/mL

ng/mL

ng/mL

O/E%

1

0

12.5

  
 

5

16.3

17.5

93.1

 

10

20.9

22.5

92.9

 

20

31.5

32.5

96.9

2

0

15.4

  
 

5

21.9

20.4

108

 

10

24.8

25.4

97.7

 

20

35.1

35.4

99.2

  1. A serum sample was fortified with 0 ng/mL, 5 ng/mL, 10 ng/mL, and 20 ng/mL recombinant MIF protein and MSIA was performed. Intact MIF concentrations were determined from accompanying standard curve.
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