Skip to main content

Table 1 Functional classification of differentially expressed proteins in normal articular cartilage and during early OA in rat identified by 2-DE and MALDI-TOF/MS

From: Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis

Spotno.a) Proteinnameb) Description Accessionno.b) Theor Mr//pIc) Exp. Mr/pId) % SeqCov.e) Match Scoref) Proteins expression compared with Normal samples (% increase)g)
Protein    Pept.  
          OA 3 days OA 5 days OA 10 days
   Extracellular Matrix          
S1 Col2α1 Collagen alpha-1 (II) chain precursor P05539 38/8.46 30-32/6.6-6.7 12 16 84 22 15 12
   Transcription, protein synthesis          
S2 Eef1α1 Elongation factor 1-alpha 1 P62630 50.11/9.1 50.11/9.1 32 13 37 499 407 314
S3 DJ-1 Protein DJ-1 88767 19.9/6.32 19.9/6.3 54 9 85 138 313 271
   Glycolysis and energy production          
S4 Nme2 Nucleoside diphosphate kinase P19804 17.28/6.9 17.3/6.9 87 16 187 127 515 700
S5 Pnp Purine nucleoside phosphorylase P85973 32.3/6.46 32.32/6.4-6.5 43 8 49 243 277 384
   Signal transduction          
S6 CaM Calmodulin P62161 16.7/4.09 16.8/5.47 63 8 101 124 130 185
S7 Pebp1 Phosphatidylethanolamine binding protein 1 P31044 20.6/5.48 17.18/4.9-5.5 52 7 76 54 92 29
   Transport          
S8 Alb Serum albumin (n-terminal fragment) P02770 65.9/5.8 68.6/6.09 24 12 105 435 853 568
S9 Hba1 Haemoglobin subunit alpha-1/2 P01946 15.1/7.93 19.7/6.32 69 6 88 231 157 284
   Others          
S10 Lxn Latexin Q64361 25.5/5.77 25/5.8 48 11 118 20 29 54
  1. a)Spot number as indicated in Figure 2.
  2. b)Protein name and accession number according UniprotKB/Swiss-prot database of Rattus norvegicus species.
  3. c)Theoretical Mr and pI according to protein sequence.
  4. d)Experimental Mr and pI calculated by analysis of the gel image with ImageMaster 2D Platinum 7.0 software.
  5. e)Sequence coverage for identified protein (peptide confidence ≥95) in relation on the full sequence.
  6. f)The Score value listed within the Query table is the Ion Score. Ion score is −10*Log (P), where P is the probability that the observed match is random event.
  7. g)% Relative volume by condition in early OA vs Normal condition (Tukey-Kramer test, p ≤0.001). The percentage of increase of the protein expression is represented by bold numbers.