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Table 1 Potential novel biomarkers for Alport syndrome

From: Effects of mycophenolate mofetil on kidney function and phosphorylation status of renal proteins in Alport COL4A3-deficient mice

Phosphospot

Phosphoprotein identified in the spot

Quantitative value (Normalized total spectra)

Molecular weight, kDa

Isoelectric point, pI

 

(Entry Name)

WT/PLC/MMF

Observed

Calculated

Observed

Basal, unphosphorylated statea

Prediction for various phosphorylation statesa

Phosphospot 1

  

17

 

9.0

  
 

Actin-related protein 2/3 complex subunit 3 (ARPC3_MOUSE)

9/13/53

 

21

 

8.78

1-18 phosphosites; 8.28-4.09

Main protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (NDUA8_MOUSE)b

35/49/53

 

20

 

8.76

1-11 phosphosites; 8.41-5.45

Phosphospot 2

  

75

 

5.5

  
 

Plastin-3 (PLST_MOUSE)

2/0/0

 

71

 

5.42

1-44 phosphosites; 5.36-4.19

 

Heat shock cognate 71 kDa protein (HSP7C_MOUSE)

2/0/0

 

71

 

5.37c

1-137 phosphosites; 5.32-2.38c

 

Adseverin (ADSV_MOUSE)

5/18/58

 

80

 

5.64

1-17 phosphosites; 5.57-4.93

Main protein

Serum albumin (ALBU_MOUSE)

33/13/99

 

69

 

5.75

1-120 phosphosites; 5.69-2.63

Phosphospot 3

  

45

 

7.5

  
 

ATP synthase subunit alpha, mitochondrial (ATPA_MOUSE)

16/0/0

 

60

 

9.22

1-79 phosphosites; 9.07-3.10

 

Catalase (CATA_MOUSE)

2/0/0

 

60

 

7.72

1-69 phosphosites; 7.19-3.73

Main protein

Glutamate dehydrogenase 1, mitochondrial (DHE3_MOUSE)

43/22/37

 

61

 

8.05

1-82 phosphosites; 7.40-3.33

Phosphospot 4

  

27

 

6.5

  
 

Serine/arginine-rich splicing factor 1 (SRSF1_MOUSE)

4/0/0

 

28

 

10.37

1-58 phosphosites; 10.22-3.08

 

26S proteasome non-ATPase regulatory subunit 9 (PSMD9_MOUSE)

4/0/0

 

25

 

6.00

1-14 phosphosites; 5.83-4.58

Main protein

Estradiol 17-beta-dehydrogenase 8 (DHB8_MOUSE)b

9/30/17

 

27

 

6.10

1-5 phosphoprotein; 5.81-5.07

Phosphospot 5

  

15

 

8.5

  
 

Destrin OS (DEST_MOUSE)

15/41/10

 

19

 

8.14

1-30 phosphosites; 7.15-3.20

 

40S ribosomal protein S14 (RS14_MOUSE)

5/0/56

 

16

 

10.07

1-15 phosphosites; 9.84-5.26

Main protein

Peptidyl-prolyl cis-trans isomerase A (PPIA_MOUSE)

72/30/44

 

18

 

7.73

1-39 phosphosites; 6.75-2.36

Phosphospot 6

  

26

 

7.0

  
 

Serine/arginine-rich splicing factor 1 (SRSF1_MOUSE)

12/1/27

 

28

 

10.37

1-58 phosphosites; 10.22-3.08

Main protein

Omega-amidase NIT2 (NIT2_MOUSE)b

49/29/34

 

31

 

6.44

1-13 phosphosites; 5.10-4.58

  1. ahttp://www.phosphosite.org; January 2014.
  2. bMain protein identified in each group, not yet described as phosphoprotein.
  3. cInformation for the human protein; March 2014.
  4. The table presents possible phosphoproteins, including the main proteins identified in this mouse model for the human Alport syndrome.
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Proteome Science

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