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Figure 4 | Proteome Science

Figure 4

From: Comparative proteomics analysis of differential proteins in respond to doxorubicin resistance in myelogenous leukemia cell lines

Figure 4

Western blotting analysis of some of differentially expressed proteins. (A) Western blotting analysis of HSP70, HSP27, and enolase 1 between K562 cells and K562/A02 cells. The amount of total protein loaded was 20 μg per lane. Differential expression of the protein of interest between doxorubicin-resistant myelogenous leukemia cell line K562/A02 and its parental control cell line K562 is normalized to actin by probing the same membrane with a monoclonal antibody specific for the protein. (B) Western blotting analysis of HSP70 and HSP27 in K562 cells with and without 1 microgram/ml doxorubicin treatment for 2 hrs, respectively.

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ISSN: 1477-5956