Interaction of peroxiredoxin V with dihydrolipoamide branched chain transacylase E2 (DBT) in mouse kidney under hypoxia

Table 1 Protein showing altered interaction by Prdx V immunoprecipitation during hypoxic stress ^a

Accession	Description	Gene	Score	MS/MS spectra	Frequency ^b
Accession	Description	Gene	Score	MS/MS spectra	Normoxia	Hypoxia
IPI00130467	Ras related protein Rab 43 isoform b	Rab43	228.7	6	ND	1/3
IPI00130535	Lipoamide acyltransferase component of branched chain α-keto acid dehydrogenase complex, mitochondial	Dbt	302.6	8	ND	2/3
IPI00131695	Serum albumin	Alb	325.3	3	1/3	3/3
IPI00225123	Non-lysosomal glucosylceramidase	Gba2	191.4	9	1/3	ND
IPI00226993	Thioredoxin	Txn1	1155.1	3	3/3	2/3
IPI00330523	Propionyl CoA carboxylase alpha chain, mitochondrial	Pcca	474.0	21	2/3	3/3
IPI00346834	Keratin type II cytoskeletal 2, oral	Krt76	184.2	7	ND	1/3
IPI00348328	Keratin Kb40	Krt78	580.5	19	1/3	ND
IPI00122281	Keratin type I cuticular Ha2	Krt32	260.3	8	1/3	ND
IPI00227140	Keratin type I cytoskeletal 14	Krt14	1399.7	16	ND	1/3
IPI00230365	Keratin type I cytoskeletal 17	Krt17	1322.1	16	ND	1/3
IPI00347019	Keratin type II cuticular Hb4	Krt84	330.8	10	ND	1/3
IPI00347096	Keratin type II cytoskeletal 72	Krt72	337.2	8	ND	1/3
IPI00420970	Keratin type II cytoskeletal 74	Krt74	1999.7	4	1/3	3/3
IPI00462140	Keratin type II cytoskeletal 1b	Krt77	2014.2	7	ND	1/3
IPI00468696	Keratin type I cytoskeletal 42	Krt42	798.6	12	ND	1/3
IPI00606510	Propionyl CoA carboxylase beta chain, mitochondrial	Pccb	266.6	10	2/3	3/3

^aProteins were affinity-purified from mouse kidneys under both normoxic and hypoxic conditions as bound interactors with Prdx V immunoprecipitation. The purified immunoprecipitates were applied to acrylamide gel-associated tryptic digestion and subjected to nano-UPLC-MS/MS for protein identification.
^bFrequency represents the number of times that the interactors were observed in three independent experiments. ND, not detected.

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