2-DE gel of the adrenal gland. A: 2-DE reference map of proteins extracted from the adrenal gland of an E19 embryo. 150 μg proteins were loaded on an 11-cm IPG strip with a linear pH gradient pI 5–8 for IEF, 12% SDS-polyacrylamide gels were used for the SDS-PAGE. Proteins were stained with Flamingo fluorescent gel stain. Identified spots were assigned a gene name. B: An overlay of the 2D gel images of the adrenal glands of E19 embryo and newborn. The identified spots are labeled with the gene names.