Proteome changes in the small intestinal mucosa of broilers (Gallus gallus) induced by high concentrations of atmospheric ammonia

Zhang, Jize; Li, Cong; Tang, Xiangfang; Lu, Qingping; Sa, Renna; Zhang, Hongfu

doi:10.1186/s12953-015-0067-4

Proteome Science

Table 3 List of differentially expressed proteins in small intestinal mucosal samples from treatment group and control group

From: Proteome changes in the small intestinal mucosa of broilers (Gallus gallus) induced by high concentrations of atmospheric ammonia

Accession ^a	Description ^b	Gene symbol	Theoretical MW ^c /pI ^d	Score ^e	Pep. no. ^f	Log ₂ fold change ^g	P- value	Biological process GO term
Energy metabolism
F1NTZ0	Uncharacterized protein (Fragment) OS = Gallus gallus GN = ADH6 PE = 3 SV = 2 - [F1NTZ0_CHICK]	ADH6	39.5/7.49	565.75	14	−0.60	0.0162	Oxidoreductase activity
R4GI36	Phosphoenolpyruvate carboxykinase, cytosolic [GTP] OS = Gallus gallus GN = PCK1 PE = 3 SV = 1 - [R4GI36_CHICK]	PCK1	61.3/7.75	64.06	2	−0.35	0.0038	Gluconeogenesis
P07322	Beta-enolase OS = Gallus gallus GN = ENO3 PE = 1 SV = 3 - [ENOB_CHICK]	ENO3	47.2/7.61	259.39	5	−0.29	0.0497	Gluconeogenesis
E1C4U7	Uncharacterized protein OS = Gallus gallus GN = NDUFB3 PE = 4 SV = 1 - [E1C4U7_CHICK]	NDUFB3	11.1/9.82	35.42	1	0.39	0.0179	Electron transport chain
E1BQJ6	Uncharacterized protein OS = Gallus gallus PE = 3 SV = 2 - [E1BQJ6_CHICK]		257.7/5.17	23.38	1	0.87	0.0490	ATP binding
Q9YHT1	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial OS = Gallus gallus GN = SDHA PE = 1 SV = 2 - [SDHA_CHICK]	SDHA	72.9/7.08	773.5	18	1.02	0.0211	Succinate dehydrogenase (ubiquinone) activity
F1NZI4	Uncharacterized protein OS = Gallus gallus GN = ATHL1 PE = 4 SV = 2 - [F1NZI4_CHICK]	ATHL1	77/5.73	30.07	1	1.15	0.0239	Catalytic activity
Lipid metabolism
Q2MJT5	Fatty acid translocase OS = Gallus gallus PE = 2 SV = 1 - [Q2MJT5_CHICK]	CD36	52.6/8.37	110.81	4	−0.45	0.0471	Lipid uptake
E1BS15	Uncharacterized protein OS = Gallus gallus GN = ACSF2 PE = 4 SV = 2 - [E1BS15_CHICK]	ACSF2	68.7/8.7	441.08	15	−0.33	0.0192	Fatty acids catalytic activity
Amino acid metabolism
P00368	Glutamate dehydrogenase 1, mitochondrial OS = Gallus gallus GN = GLUD1 PE = 1 SV = 1 - [DHE3_CHICK]	GLUD1	55.7/8.28	641.29	19	−0.50	0.0155	Glutamate dehydrogenase [NAD(P)+] activity
F1P3F9	Glutamate dehydrogenase OS = Gallus gallus GN = GLUD1 PE = 3 SV = 2 - [F1P3F9_CHICK]	GLUD1	47.6/8.18	695.44	19	−0.39	0.0288	Glutamate dehydrogenase [NAD(P)+] activity
Vitamin metabolism
F1P4K4	Uncharacterized protein OS = Gallus gallus GN = ALDH8A1 PE = 3 SV = 2 - [F1P4K4_CHICK]	ALDH8A1	53.2/7.83	31.26	1	−0.54	0.0080	9-cis-retinoic acid biosynthetic process
Cell cytoskeleton
Q90WF1	Filamin OS = Gallus gallus PE = 2 SV = 1 - [Q90WF1_CHICK]	FLNA	272.8/6.35	103.29	2	−0.72	0.0018	Actin-binding
D2Z1L9	LIM and SH3 protein 1 OS = Gallus gallus GN = LASP1 PE = 2 SV = 1 - [D2Z1L9_CHICK]	LASP1	29.6/7.4	417.26	11	−0.43	0.0284	Zinc ion binding
Cell growth and proliferation
R4GF89	Uncharacterized protein OS = Gallus gallus GN = SFN PE = 3 SV = 1 - [R4GF89_CHICK]	SFN	27.7/5.01	114.99	3	−0.54	0.0239	Positive regulation of cell growth
E1B	Uncharacterized protein OS = Gallus	RP	14.9/1	286.	8	−0.46	0.0	Structural
Y89	gallus GN = RPL23 PE = 3 SV = 2 - [E1BY89_CHICK]	L23	0.51	2			019	constituent of ribosome
F1NQS9	Uncharacterized protein OS = Gallus gallus GN = ZNF598 PE = 4 SV = 2 - [F1NQS9_CHICK]	ZNF598	99.6/8.57	28.5	1	−0.42	0.0041	Zinc ion binding
E1BTA6	Uncharacterized protein OS = Gallus gallus GN = SEPT12 PE = 3 SV = 2 - [E1BTA6_CHICK]	SEPT12	47.1/8.68	52.61	2	−0.33	0.0202	GTP binding
Transcriptional and translational regulation
F1P0C0	Uncharacterized protein OS = Gallus gallus GN = HMGN1 PE = 4 SV = 1 - [F1P0C0_CHICK]	HMGN1	11.1/9.26	68.36	1	−0.55	0.0223	Chromatin binding
E1C9F0	Uncharacterized protein OS = Gallus gallus GN = DYNC2H1 PE = 4 SV = 2 - [E1C9F0_CHICK]	DYNC2H1	491.7/6.43	22.2	1	−0.50	0.0062	Protein processing
E1BT82	Uncharacterized protein OS = Gallus gallus GN = EIF2S2 PE = 2 SV = 1 - [E1BT82_CHICK]	EIF2S2	37.9/6.13	71.23	4	−0.44	0.0040	Translation initiation factor activity
Q5ZJ39	Density-regulated protein OS = Gallus gallus GN = DENR PE = 2 SV = 1 - [DENR_CHICK]	DENR	22.1/5.21	60.49	1	−0.41	0.0080	Translation initiation factor activity
F1NS60	Uncharacterized protein (Fragment) OS = Gallus gallus GN = MMS19 PE = 4 SV = 2 - [F1NS60_CHICK]	MMS19	111.8/5.99	55.46	1	−0.38	0.0038	Transcription coactivator activity
E1C4N0	Uncharacterized protein OS = Gallus gallus GN = RPS10 PE = 4 SV = 2 - [E1C4N0_CHICK]	RPS10	18.9/10.15	183.89	6	−0.36	0.0068	Structural constituent of ribosome
R4GL23	Uncharacterized protein OS = Gallus gallus GN = CHTOP PE = 4 SV = 1 - [R4GL23_CHICK]	CHTOP	26.3/12.23	83.09	2	−0.30	0.0036	Transcription export complex
F1NLT8	Uncharacterized protein (Fragment) OS = Gallus gallus GN = ARHGDIB PE = 4 SV = 1 - [F1NLT8_CHICK]	ARHGDIB	23.2/5.2	235.16	7	−0.29	0.0435	Rho GDP-dissociation inhibitor activity
F1NA55	Eukaryotic translation initiation factor 2A OS = Gallus gallus GN = EIF2A PE = 2 SV = 2 - [F1NA55_CHICK]	EIF2A	62.7/8.79	62.63	1	0.27	0.0449	Translation initiation factor activity
Q800W4	TIA-1 OS = Gallus gallus GN = TIA1 PE = 2 SV = 1 - [Q800W4_CHICK]	TIA1	41.3/7.72	62.06	1	0.29	0.0074	Nucleotide binding
Immune response
P40618	High mobility group protein B3 OS = Gallus gallus GN = HMGB3 PE = 2 SV = 3 - [HMGB3_CHICK]	HMGB3	23/8.12	126.17	4	−0.59	0.0009	DNA binding
Q9YH06	High mobility group protein B1 OS = Gallus gallus GN = HMGB1 PE = 1 SV = 1 - [HMGB1_CHICK]	HMGB1	24.9/5.74	165.42	6	−0.44	0.0084	DNA binding
E1C9I0	Unconventional myosin-Ig OS = Gallus gallus GN = MYO1G PE = 4 SV = 2 - [E1C9I0_CHICK]	MYO1G	115/8.78	203.91	7	−0.47	0.0410	Nucleotide-binding(motor activity)
E1BTE2	Uncharacterized protein OS = Gallus gallus GN = SERPINB5 PE = 3 SV = 2 - [E1BTE2_CHICK]	SERPINB5	42.6/5.96	218.9	7	−0.40	0.0017	Serine-type endopeptidase inhibitor activity
Q90643	Interferon regulatory factor 3 OS = Gallus gallus GN = IRF3 PE = 2 SV = 1 - [IRF3_CHICK]	IRF3	54.4/5.21	45.52	2	−0.33	0.0460	Activation of innate immune response
E1BWS0	Uncharacterized protein OS = Gallus gallus GN = GIT2 PE = 4 SV = 2 - [E1BWS0_CHICK]	GIT2	84.5/6.98	29.12	1	−0.32	0.0461	ARF GTPase activator activity
E1BUY6	Uncharacterized protein OS = Gallus gallus GN = HMHA1 PE = 4 SV = 2 - [E1BUY6_CHICK]	HMHA1	103.3/7.97	29.79	1	−0.31	0.0080	GTPase activator activity
E1BVP2	Uncharacterized protein OS = Gallus gallus GN = PLD1 PE = 4 SV = 2 - [E1BVP2_CHICK]	PLD1	118.9/8.98	50.12	2	−0.27	0.0397	Defense response to Gram-positive bacterium
Apoptosis
I3VQH4	Interleukin enhancer binding factor 3-like protein OS = Gallus gallus GN = ILF3 PE = 2 SV = 1 - [I3VQH4_CHICK]	ILF3	95/8.81	68.88	3	0.30	0.0025	Participate in the apoptosis
R4GLP0	Uncharacterized protein OS = Gallus gallus GN = COX7C PE = 4 SV = 1 - [R4GLP0_CHICK]	COX7C	7.2/10.96	27.81	1	0.55	0.0380	Cytochrome-c oxidase activity
F1NIC5	Uncharacterized protein (Fragment) OS = Gallus gallus GN = TIMM8A PE = 4 SV = 2 - [F1NIC5_CHICK]	TIMM8A	12.2/7.78	135.92	4	0.57	0.0346	Protein transport (metal ion binding)
Oxidative stress
H9L201	Uncharacterized protein OS = Gallus gallus GN = PNKD PE = 3 SV = 2 - [H9L201_CHICK]	PNKD	48/9.35	31.96	1	−0.46	0.0095	Glutathione biosynthetic process
F1NQC3	Glutamine synthetase OS = Gallus gallus GN = GLUL PE = 3 SV = 2 - [F1NQC3_CHICK]	GLUL	46.5/7.72	479.53	13	−0.40	0.0483	Glutamate-ammonia ligase activity
P08267	Ferritin heavy chain OS = Gallus gallus GN = FTH PE = 2 SV = 2 - [FRIH_CHICK]	FTH	21.1/6.21	311.58	8	0.34	0.0248	Ferroxidase activity
Neurotoxicity
F1P4B2	Protein piccolo (Fragment) OS = Gallus gallus GN = PCLO PE = 4 SV = 2 - [F1P4B2_CHICK]	LOC768552	560.2/6.77	33.63	1	0.62	0.0247	cAMP-mediated signaling

^aUniprot_Gallus gallus_9031 database accession number.
^bThe name of the protein exclusive of the identifier that appears in the database.
^cTheoretical molecular mass (kDa).
^dTheoretical pI.
^eThe sum of the scores of the individual peptides.
^fThe number of distinct peptide sequences in the protein group.
^gDifferential protein expression in the treatment group was presented as a log₂ fold change relative to the control group.

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