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Figure 2 | Proteome Science

Figure 2

From: Proteomic study of different culture medium serum volume fractions on RANKL-dependent RAW264.7 cells differentiating into osteoclasts

Figure 2

Comparison of TRAP-positive cell numbers and size of osteoclasts formed in medium supplemented with 10% FBS and 1% FBS. (A): significantly more osteoclasts were observed in 1% FBS medium on Days 4, 5, 6 and 9; (B): the number of osteoclasts reached a maximum in 10% FBS medium on Day 3, after which the numbers declined dramatically, while the number of osteoclasts reached a maximum in 1% FBS medium on Day 4 and subsequently decreased moderately. (C): Osteoclasts formed in 10% FBS medium were obviously larger on Days 3, 4, 5 and 6, while osteoclasts formed in 1% FBS medium were significantly larger on Day 7. (D): Osteoclasts formed in 10% FBS medium reached maximum size on Day 6, while osteoclasts formed in 1% FBS medium reached maximum size on Day 7. *: P < 0.05.

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Proteome Science

ISSN: 1477-5956