Differential proteins among normal cervix cells and cervical cancer cells with HPV-16 infection, through mass spectrometry-based Proteomics (2D-DIGE) in women from Southern México

Table 2 Characteristics of cervical tissues samples of comparatives groups CC/HPV-16 and control without SIL and HPV infection

Tests^a	Pool CC/HPV-16	Pool without SIL and HPV infection (control)
Background	Cytological^b study: Squamous cell carcinoma Colposcopic examination: cervical carcinoma Clinical diagnosis: 4 cases stage IIB, one stage IB1 and 1 case stage IB2	Cytological^b study: normal cells with reactive changes to nonspecific inflammatory processes Normal colposcopy
Section 1 (paraffin embedding) - Histopathological diagnosis (H-E) - in situ hybridization with tyramide amplification - Immunohistochemistry for Ki-67	Squamous Cell Carcinoma Positive of HR- HPV Positive in large pleomorphic nuclei of malignant squamous cells form irregular nests invading the stroma	Negative Negative Positive in nuclei of parabasal cells in basal layer
Section 2 - INNO-LIPA extra (Genotyping of HVP)	HPV-16	Negative of HPV infection
Section 3 - Extraction of proteins for 2D- DIGE and MALDI-TOF	Increased expression in cervical cancer Mimecan, Actin from aortic smooth muscle and Lumican. Decreased expression: Keratin, type II cytoskeletal 5, Peroxiredoxin-1 and 14-3-3 protein sigma

^aEach sample of tissue fragment into three sections and all tests were performed, six samples for group CC / HPV-16 and four samples were used for the control group. For more details see section Methods
^b Without coninfection by Trichomonas vaginalis, Candida sp, Actinomyces sp, Herpes simplex virus according to the Bethesda 2001 system

ISSN: 1477-5956