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Table 1 Proteins identified in methanol extract by LC-MS/MS. Proteins with a single peptide are considered “tentatively identified”

From: Protein profiles of hatchery egg shell membrane

  Accession Protein MW [kDa] Scores # Peptides
1 ENSGALP00000036403 Ovalbumin 42.9 629.9 11
2 ENSGALP00000005544 Ovomucoid 22.6 338.1 7
3 ENSGALP00000017755 Ovocleidin-116 76.8 225 2
4 ENSGALP00000008163 Orosomucoid 1 (ovoglycoprotein) precursor 22.3 167 3
5 ENSGALP00000042635 peptidyl-prolyl cis-trans isomerase FKBP1A 8.9 163.5 3
6 ENSGALP00000016177 Lysozyme C 16.2 129.2 2
7 ENSGALP00000016632 keratin 8, type II 42.1 119.1 2
8 ENSGALP00000031725 SH3 domain binding glutamic acid-rich protein like (SH3BGRL), mRNA 12.9 109.6 2
9 ENSGALP00000027483 Ubiquitin-fold modifier 1 9 102.1 1
10 ENSGALP00000035930 Gallinacin-9 7.3 85.7 2
11 ENSGALP00000010763 Ovocalyxin-36 precursor 58.3 83.7 1
12 ENSGALP00000019758 Diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) (DBI), mRNA. 9.6 69.8 2
13 ENSGALP00000026846 Gallinacin-10 7.1 62.6 2
14 ENSGALP00000006093 Keratin, type I cytoskeletal 19 46 62.3 3
15 ENSGALP00000009976 Usher syndrome 1C 100.1 51.9 1
16 ENSGALP00000012729 Uncharacterized protein 21 50.9 1
17 ENSGALP00000040654 Thymosin, beta 4 5 47.8 2
18 ENSGALP00000043411 Collagen, type XVI, alpha 1 156.6 42 1
19 ENSGALP00000002523 Signal peptidase complex subunit 1 homolog 27.2 37.9 1
20 ENSGALP00000013908 Zinc finger BED domain-containing protein 4 132.4 37.8 2
21 ENSGALP00000038912 Alpha-D-globin (HBAD), mRNA. 15.7 37.3 1
22 ENSGALP00000019988 Utrophin 398.6 36.5 2
23 ENSGALP00000000876 Fatty acid-binding protein, heart 14.8 34.6 1
24 ENSGALP00000026777 Nociceptin precursor 21.4 34.4 1
25 ENSGALP00000026863 Polycystic kidney and hepatic disease 1 (autosomal recessive) 440 32.7 1
26 ENSGALP00000018601 Serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor) 6 26.7 1
27 ENSGALP00000025439 Probable arginyl-tRNA synthetase, mitochondrial 65.3 25.2 1
28 ENSGALP00000039913 Transforming, acidic coiled-coil containing protein 1 86.4 24.5 2
29 ENSMGAP00000001722 elaC ribonuclease Z 2 94.2 24.2 1
30 ENSGALP00000031519 Fibroblast growth factor 2 16.2 22.8 1
31 ENSGALP00000039675 Nuclear receptor coactivator 2 44.7 21.5 1
32 ENSGALP00000000325 WD repeat-containing protein 36 98.2 21.2 1
33 ENSGALP00000019412 Septin 3 40 20.5 1
34 ENSGALP00000027541 High mobility group protein B1 24.9 20 1
35 ENSGALP00000014919 tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6 54.2 19.8 1
36 ENSGALP00000043133 Fibrinogen silencer binding protein 36.4 19 1
37 ENSGALP00000000726 SH3 domain binding glutamate-rich protein like 3 10.5 18.8 1
38 ENSGALP00000038283 UPF3 regulator of nonsense transcripts homolog B (yeast) 56.9 18.4 1
39 ENSGALP00000000275 Mutated in colorectal cancers 112.6 18.3 1
40 ENSGALP00000038735 Polyubiquitin-B Ubiquitin 109.6 15.5 1
41 ENSGALP00000040476 Neuregulin 2 65.5 15 1
  1. The underlined proteins also occur in guanidine HCl extract (Additional file 1: Table S1)