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Table 1 Proteins identified in methanol extract by LC-MS/MS. Proteins with a single peptide are considered “tentatively identified”

From: Protein profiles of hatchery egg shell membrane

 

Accession

Protein

MW [kDa]

Scores

# Peptides

1

ENSGALP00000036403

Ovalbumin

42.9

629.9

11

2

ENSGALP00000005544

Ovomucoid

22.6

338.1

7

3

ENSGALP00000017755

Ovocleidin-116

76.8

225

2

4

ENSGALP00000008163

Orosomucoid 1 (ovoglycoprotein) precursor

22.3

167

3

5

ENSGALP00000042635

peptidyl-prolyl cis-trans isomerase FKBP1A

8.9

163.5

3

6

ENSGALP00000016177

Lysozyme C

16.2

129.2

2

7

ENSGALP00000016632

keratin 8, type II

42.1

119.1

2

8

ENSGALP00000031725

SH3 domain binding glutamic acid-rich protein like (SH3BGRL), mRNA

12.9

109.6

2

9

ENSGALP00000027483

Ubiquitin-fold modifier 1

9

102.1

1

10

ENSGALP00000035930

Gallinacin-9

7.3

85.7

2

11

ENSGALP00000010763

Ovocalyxin-36 precursor

58.3

83.7

1

12

ENSGALP00000019758

Diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) (DBI), mRNA.

9.6

69.8

2

13

ENSGALP00000026846

Gallinacin-10

7.1

62.6

2

14

ENSGALP00000006093

Keratin, type I cytoskeletal 19

46

62.3

3

15

ENSGALP00000009976

Usher syndrome 1C

100.1

51.9

1

16

ENSGALP00000012729

Uncharacterized protein

21

50.9

1

17

ENSGALP00000040654

Thymosin, beta 4

5

47.8

2

18

ENSGALP00000043411

Collagen, type XVI, alpha 1

156.6

42

1

19

ENSGALP00000002523

Signal peptidase complex subunit 1 homolog

27.2

37.9

1

20

ENSGALP00000013908

Zinc finger BED domain-containing protein 4

132.4

37.8

2

21

ENSGALP00000038912

Alpha-D-globin (HBAD), mRNA.

15.7

37.3

1

22

ENSGALP00000019988

Utrophin

398.6

36.5

2

23

ENSGALP00000000876

Fatty acid-binding protein, heart

14.8

34.6

1

24

ENSGALP00000026777

Nociceptin precursor

21.4

34.4

1

25

ENSGALP00000026863

Polycystic kidney and hepatic disease 1 (autosomal recessive)

440

32.7

1

26

ENSGALP00000018601

Serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)

6

26.7

1

27

ENSGALP00000025439

Probable arginyl-tRNA synthetase, mitochondrial

65.3

25.2

1

28

ENSGALP00000039913

Transforming, acidic coiled-coil containing protein 1

86.4

24.5

2

29

ENSMGAP00000001722

elaC ribonuclease Z 2

94.2

24.2

1

30

ENSGALP00000031519

Fibroblast growth factor 2

16.2

22.8

1

31

ENSGALP00000039675

Nuclear receptor coactivator 2

44.7

21.5

1

32

ENSGALP00000000325

WD repeat-containing protein 36

98.2

21.2

1

33

ENSGALP00000019412

Septin 3

40

20.5

1

34

ENSGALP00000027541

High mobility group protein B1

24.9

20

1

35

ENSGALP00000014919

tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6

54.2

19.8

1

36

ENSGALP00000043133

Fibrinogen silencer binding protein

36.4

19

1

37

ENSGALP00000000726

SH3 domain binding glutamate-rich protein like 3

10.5

18.8

1

38

ENSGALP00000038283

UPF3 regulator of nonsense transcripts homolog B (yeast)

56.9

18.4

1

39

ENSGALP00000000275

Mutated in colorectal cancers

112.6

18.3

1

40

ENSGALP00000038735

Polyubiquitin-B Ubiquitin

109.6

15.5

1

41

ENSGALP00000040476

Neuregulin 2

65.5

15

1

  1. The underlined proteins also occur in guanidine HCl extract (Additional file 1: Table S1)