Table 1 Proteins differentially abundant over time as a result of the microbial challenge. The p-values for both variables: time and litter are given for each spot, and a list of proteins identified by mass spectrometry, together with theoretical molecular weights, isoelectric points, accession number and Mascot score
From: The effect of microbial challenge on the intestinal proteome of broiler chickens
Spot | Protein | P- value Litter | P-value Time | Accession | Theoretical MW (kDa)/ Pi | Mascot score |
|---|---|---|---|---|---|---|
Proteins that showed decreased abundance over time in challenged birds | ||||||
534 | Cytochrome b-c1 complex subunit 1, mitochondrial Alpha-enolase | 0.042 | 0.042 | gi|507543754 gi|6048768 | 53.4/6.58 47.6/6.17 | 910 641 |
530 | Heat shock cognate 71 kDa protein Heat shock 70 kDa protein | 0.041 | 0.0082 | gi|45384370 gi|55742654 | 71.0/5.47 69.9/5.53 | 1504 812 |
30 | Protein disulfide-isomerase A3 precursor dihydropyrimidinase-related protein /Collapsin response mediator protein CRMP-62 | 0.041 | 0.0057 | gi|45383890 gi|3122036 | 56.5/5.76 62.7/5.96 | 1272 486 |
523 | Adseverin | 0.041 | 0.0033 | gi|347360989 | 81.1/5.53 | 519 |
556 | Collagen alpha-2(VI) chain precursor Collagen alpha-1(VI) chain precursor l-caldesmon | 0.041 | 0.0067 | gi|45384382 gi|49225581 gi|212243 | 110.3/5.66 109.0/5.63 59.0/8.66 | 1038 490 287 |
513 | Transitional endoplasmic reticulum ATPase | 0.041 | 0.0040 | gi|113206112 | 90.0/5.14 | 261 |
11 | Heat shock protein 70 Heat shock cognate 71 kDa protein | 0.043 | 0.012 | gi|30962014 gi|45384370 | 70.1/5.66 71.0/5.47 | 599 307 |
522 | Collagen alpha-1(VI) chain precursor | 0.039 | 0.0033 | gi|49225581 | 109.0/5.63 | 532 |
24 | T-complex protein 1 subunit epsilon | 0.046 | 0.0044 | gi|60302774 | 60.2/5.53 | 941 |
88 | B-creatine kinase | 0.041 | 0.037 | gi|211235 | 42.5/5.78 | 937 |
12 | Annexin A6 NADPH--cytochrome P450 reductase | 0.041 | 0.017 | gi|50982399 gi|307775405 | 75.6/5.57 77.3/5.42 | 1336 528 |
33 | Protein disulfide-isomerase A3 precursor Plastin-3 | 0.039 | 0.0033 | gi|453838905 gi|7530180 | 56.5/5.76 71.3/5.51 | 2873 97 |
77 | Aminoacylase-1-like isoform 1 Protein disulfide-isomerase A3 precursor | 0.046 | 0.0057 | gi|363738588 gi|45383890 | 46.3/5.98 56.5/5.76 | 442 424 |
29 | Tubulin alpha ATP synthase subunit beta, mitochondrial Protein disulfide-isomerase A3 precursor | 0.041 | 0.0066 | gi|223280 gi|71897237 gi|5383890 | 46.3/5.0 56.7/5.59 56.5/5.76 | 451 140 135 |
509 | Heat shock protein 108 Endoplasmin precursor | 0.041 | 0.0067 | gi|194220334 gi|45383562 | 91.4/4.98 91.7/4.83 | 524 523 |
520 | Adenosine deaminase | 0.041 | 0.0044 | gi|57529377 | 41.1/5.82 | 1131 |
Proteins that showed increased abundance over time in challenged birds | ||||||
133 | Smooth muscle gamma actin Beta-actin Actin, alpha skeletal muscle B isoform 2 | 0.041 | 0.017 | gi|2967678 gi|63018 gi|50803534 | 42.3/5.31 42.1/5.29 42.4/5.16 | 310 262 247 |
220 | Calcium-binding protein p22 | 0.041 | 0.0057 | gi|56118996 | 22.5/4.97 | 345 |
40 | Histone-binding protein RBBP4 | 0.041 | 0.029 | gi|45382339 | 47.9/4.74 | 56 |
84 | Tropomyosin alpha-1 chain | 0.041 | 0.023 | gi|45382323 | 32.9/4.73 | 484 |
102 | Desmin | 0.041 | 0.0044 | gi|2959450 | 51.7/5.30 | 330 |
552 | Desmin Smooth muscle gamma actin Actin, cytoplasmic type 5 ATP synthase subunit beta, mitochondrial precursor Vimentin Heat shock cognate 71 kDa protein | 0.041 | 0.0033 | gi|2959450 gi|2967678 gi|56119084 gi|71897237 gi|114326309 gi|45384370 | 51.7/5.30 42.3/5.31 42.2/5.3 56.7/5.59 53.2/5.09 71.0/5.47 | 1193 1002 937 218 133 132 |
502 | Apolipoprotein A-I | 0.043 | 0.029 | gi|211159 | 30.7/5.58 | 1020 |
550 | Alpha-enolase Hypothetical protein CJMB04_24e12 26S protease regulatory subunit7 Beta-enolase | 0.046 | 0.0044 | gi|46048768 gi|53135040 gi|57525333 gi|46048765 | 47.6/6.11 23.8/6.79 49.0/5.72 47.6/7.28 | 872 555 331 137 |
546 | Actin, aortic smooth muscle | 0.041 | 0.00444 | gi|71895043 | 42.4/5.23 | 1063 |
73 | Beta-actin Smooth muscle gamma actin | 0.046 | 0.023 | gi|63018 gi|2967678 | 42.1/5.29 42.3/5.31 | 263 256 |
Proteins that show variable, yet significant changes in abundance over time in challenged birds | ||||||
134 | PREDICTED:pyridoxal kinase | 0.048 | 0.0066 | gi|27465053 | 32.8/4.65 | 2136 |
126 | Alpha-tropomyosin 2 | 0.046 | 0.0066 | 363728772 | 35.2/5.93 | 244 |