Fig. 5
Gene Ontology (GO) terms and pathway analysis of sheep proteins identified by Mascot database search engine. Protein data were derived from combined in-gel and in-solution workflows of serum protein samples from healthy sheep aligned to Bos taurus gene entries. Up to 349 genes were resolved from a total number of 379 sheep protein identifications (IDs) by the Protein Analysis THrough Evolutionary Relationships (PANTHER) classification tool [68]. The GO domain of molecular function had 264 function hits (a); biological process had 586 process hits (b); cellular component had 214 component hits (c); protein class had 386 class hits (d). There were 127 protein pathway hits with 49 prominent pathways, but only 25 are displayed (e). The other 24 protein pathway names not displayed with their codes and percentage contribution in parenthesises were: Axon guidance mediated by Slit/Robo (P00008)(0.80%), Beta1 adrenergic receptor signalling pathway (P04377)(0.80%), JAK/STAT signalling pathway (P00038) (0.80%), Ionotropic glutamate receptor pathway (P00037)(0.80%), Alzheimer disease-amyloid secretase pathway (P00003)(0.80%), Phenylethylamine degradation (P02766)(0.80%), Phenylalanine biosynthesis (P02765)(0.80%), Inflammation mediated by chemokine and cytokine signalling pathway (P00031)(4.70%), Asparagine and aspartate biosynthesis (P02730)(0.80%), Huntington disease (P00029)(2.40%), Heterotrimeric G-protein signalling pathway-Gq alpha and Go alpha mediated pathway (P00027)(0.80%), Wnt signalling pathway (P00057)(5.50%), Glycolysis (P00024)(0.80%), General transcription regulation (P00023)(0.80%), T cell activation (P00053)(1.60%), TGF-beta signalling pathway (P00052)(0.80%), Tyrosine biosynthesis (P02784) (0.80%), Plasminogen activating cascade (P00050) (3.10%), Endothelin signalling pathway (P00019)(0.80%), DNA replication (P00017)(0.80%), Cytoskeletal regulation by Rho GTPase (P00016)(1.60%), Nicotinic acetylcholine receptor signalling pathway (P00044)(2.40%), B cell activation (P00010)(2.40%) and CCKR signalling map (P06959)(2.40%)