Fig. 4

Gentamicin induced cell apoptosis is dose dependent. Box Plot graphs obtained from flow cytometry analysis of fluorescently labeled Annexin V and propidium Iodide (PI) SL pericytes. Cells were incubated for 24 h at increasing concentration of GTM (1, 5 and 10 mg/ml). a The percentage of live cells population showing a negative signal for either Annexin or PI staining, decreased significantly at the GTM concentration of 5 mg/ml (p < 0.049) and 10 mg/ml (p < 0.00079). b The Annexin positive PI negative population showed no significance after 24 h of GTM incubation at any of the GTM concentrations used. c Cell treated with 10 mg/ml GTM showed a marked significance (p < 0.0025) for late stage apoptosis. Data consisted of 4 independent experiments for controls and 3 independent experiment for GTM treated cell. Differences between controls and treated cells were analyzed with one-way ANOVA followed by a Dunnett’s test (9 degrees of freedom) when data showed significance. Differences were considered significant for p < 0.05. Statistical test were performed with R version 3.3.1 (R core team) *p < 0.05, **p < 0.01 ***p < 0.001