Fig. 2
From: Profiling the interactome of protein kinase C ζ by proteomics and bioinformatics
Isolation of PKCζ interacting proteins. a Western blotting analysis of PKCζ immunoprecipitates. MBA-MD-231 cells were transfected with vector control and Flag-PKCζ plasmids, and stable clones were cultured. The presence of Flag and PKCζ in these cells were detected by Western blotting. Co-IP was performed using anti-Flag conjugated beads. b SDS-PAGE separation of the Flag-PKCζ immunoprecipitates. The gel was visualized using silver staining