Fig. 6
From: Profiling the interactome of protein kinase C ζ by proteomics and bioinformatics
The interaction between PKCζ and PPP2CA. a The mRNA levels of PPP2CA and PKCζ in breast cancer samples from the TCGA database. b Western blotting analysis of the PPP2CA immunoprecipitates from MDA-MB-231 and MCF-7 cells. Co-IP was performed using anti-PPP2CA antibody conjugated protein G agarose beads. Normal IgG was used as control. c Immunofluorescence analysis of PKCζ and PPP2CA in MDA-MB-231 cells. Cell nuclei were stained with DAPI. d PPP2CA knockdown in MDA-MB-231 cell enhanced cell migration. The efficiency of PPP2CA knockdown was examined by qRT-PCR and Western blotting. Bar; mean; error bar: SD (*P < 0.05, **P < 0.01, ***P < 0.0001, by student’s t-test)