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Table 1 Protein expression profile by comparison of No treatment and FA treatment of HepG2)

From: Hepatic protein Carbonylation profiles induced by lipid accumulation and oxidative stress for investigating cellular response to non-alcoholic fatty liver disease in vitro

Spot No. Protein ID Protein name 2DE Prediction Mascot score Sequence coverage Log2 Ratio*
MW(kDa) pI MW(kDa) pI
1 O94925 Glutaminase kidney isoform, mitochondrial 57 6.9 73.4 7.85 88 9 1.06
2 P78417 Glutathione S-transferase omega-1 26 5.8 27.5 6.24 261 29 1.28
3 Q15365 Poly(rC)-binding protein 1 39 7.1 37.5 6.66 349 32 2.03
4 P00558 Phosphoglycerate kinase 42 8.8 44.6 8.3 216 20 −1.58
5 Q9NUB1 Acetyl-coenzyme A synthetase 2-like, mitochondrial 75 7.0 74.8 6.66 901 38 1.02
6 P28838 Cytosol aminopeptidase 49 7.5 56.1 8.03 116 9 1.22
7 P30740 Leukocyte elastase inhibitor 41 6.3 42.7 5.9 146 20 1.00
8 P21796 Voltage-dependent anion-selective channel protein 1 31 8.3 30.7 8.62 29 7 1.12
9 P30084 Enoyl-CoA hydratase, mitochondrial 25 6.3 31.3 8.34 37 21 1.06
10 P37837 Transaldolase 35 6.0 37.5 6.36 171 10 1.27
11 P11142 Heat shock cognate 71 kDa protein 77 5.6 70.8 5.37 163 16 1.50
12 Q562R1 Beta-actin-like protein 2 303 4.3 40.0 5.39 33 4 −1.15
13 O57874 Isocitrate dehydrogenase [NADP] cytoplasmic 45 7.7 46.6 6.53 1168 60 1.99
14 O00217 NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial 22 4.2 23.7 6.00 34 4 1.31
15 O43933 Peroxisome biogenesis factor 1 128 5.9 14.2 5.91 41 2 1.00
16 P00338 L-lactate dehydrogenase A chain isoform 3 33 8.8 36.6 8.44 1732 80.1 −2.00
17 P02771 Alpha-fetoprotein 73 5.3 68.6 5.48 63 5 1.13
18 Q92890 Ubiquitin fusion degradation protein 1 homolog 37 7.1 34.5 6.27 109 20 2.38
19 P06733 Alpha-enolase 47 8.5 47.1 7.01 421 35 2.43
  1. *Log2 Ratio refers to Log2 ratio of spot intensity from FA treatment to No treatment