Table 1 Protein expression profile by comparison of No treatment and FA treatment of HepG2)
Spot No. | Protein ID | Protein name | 2DE | Prediction | Mascot score | Sequence coverage | Log2 Ratio* | ||
|---|---|---|---|---|---|---|---|---|---|
MW(kDa) | pI | MW(kDa) | pI | ||||||
1 | O94925 | Glutaminase kidney isoform, mitochondrial | 57 | 6.9 | 73.4 | 7.85 | 88 | 9 | 1.06 |
2 | P78417 | Glutathione S-transferase omega-1 | 26 | 5.8 | 27.5 | 6.24 | 261 | 29 | 1.28 |
3 | Q15365 | Poly(rC)-binding protein 1 | 39 | 7.1 | 37.5 | 6.66 | 349 | 32 | 2.03 |
4 | P00558 | Phosphoglycerate kinase | 42 | 8.8 | 44.6 | 8.3 | 216 | 20 | −1.58 |
5 | Q9NUB1 | Acetyl-coenzyme A synthetase 2-like, mitochondrial | 75 | 7.0 | 74.8 | 6.66 | 901 | 38 | 1.02 |
6 | P28838 | Cytosol aminopeptidase | 49 | 7.5 | 56.1 | 8.03 | 116 | 9 | 1.22 |
7 | P30740 | Leukocyte elastase inhibitor | 41 | 6.3 | 42.7 | 5.9 | 146 | 20 | 1.00 |
8 | P21796 | Voltage-dependent anion-selective channel protein 1 | 31 | 8.3 | 30.7 | 8.62 | 29 | 7 | 1.12 |
9 | P30084 | Enoyl-CoA hydratase, mitochondrial | 25 | 6.3 | 31.3 | 8.34 | 37 | 21 | 1.06 |
10 | P37837 | Transaldolase | 35 | 6.0 | 37.5 | 6.36 | 171 | 10 | 1.27 |
11 | P11142 | Heat shock cognate 71 kDa protein | 77 | 5.6 | 70.8 | 5.37 | 163 | 16 | 1.50 |
12 | Q562R1 | Beta-actin-like protein 2 | 303 | 4.3 | 40.0 | 5.39 | 33 | 4 | −1.15 |
13 | O57874 | Isocitrate dehydrogenase [NADP] cytoplasmic | 45 | 7.7 | 46.6 | 6.53 | 1168 | 60 | 1.99 |
14 | O00217 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial | 22 | 4.2 | 23.7 | 6.00 | 34 | 4 | 1.31 |
15 | O43933 | Peroxisome biogenesis factor 1 | 128 | 5.9 | 14.2 | 5.91 | 41 | 2 | 1.00 |
16 | P00338 | L-lactate dehydrogenase A chain isoform 3 | 33 | 8.8 | 36.6 | 8.44 | 1732 | 80.1 | −2.00 |
17 | P02771 | Alpha-fetoprotein | 73 | 5.3 | 68.6 | 5.48 | 63 | 5 | 1.13 |
18 | Q92890 | Ubiquitin fusion degradation protein 1 homolog | 37 | 7.1 | 34.5 | 6.27 | 109 | 20 | 2.38 |
19 | P06733 | Alpha-enolase | 47 | 8.5 | 47.1 | 7.01 | 421 | 35 | 2.43 |