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Table 2 Protein expression profile by comparison of No treatment and FA/MND treatment of HepG2

From: Hepatic protein Carbonylation profiles induced by lipid accumulation and oxidative stress for investigating cellular response to non-alcoholic fatty liver disease in vitro

Spot No. Protein ID Protein name 2DE Prediction Mascot score Sequence coverage Log2 Ratio*
MW(kDa) pI MW(kDa) pI
1 O94925 Glutaminase kidney isoform, mitochondrial 57 6.9 73.4 7.85 88 9 1.22
6 P28838 Cytosol aminopeptidase 49 7.5 56.1 8.03 116 9 1.05
12 Q562R1 Beta-actin-like protein 2 303 4.3 40.0 5.39 33 4 −1.29
16 P00338 L-lactate dehydrogenase A chain isoform 3 33 8.8 36.6 8.44 1732 80.1 −1.40
19 P06733 Alpha-enolase 47 8.5 47.1 7.01 421 35 1.59
20 Q06830 Peroxiredoxin-1 22 8.4 22.1 8.27 545 55 2.63
21 P16930 Fumarylacetoacetase 41 7.6 46.3 6.46 118 6 1.01
22 P68104 Elongation factor 1-alpha 1 50 9.8 50.1 9.1 161 4 −2.64
23 P33176 Kinesin-1 heavy chain 137 6.7 109.6 6.12 136 7 −1.15
24 P43243 Matrin-3 129 7.5 94.6 5.87 228 19 − 1.65
25 Q13733 Sodium/potassium-transporting ATPase subunit alpha-4 57 6.5 114.1 6.23 28 2 1.17
26 Q12931 Heat shock protein 75 kDa, mitochondrial 79 7.3 80.1 8.3 60 10 1.06
27 P06733 Alpha-enolase 48 6.8 47.1 7.01 102 27 1.00
28 P61163 Alpha-centractin 43 7.3 42.6 6.19 92 8 −1.00
29 Q15365 Poly(rC)-binding protein 1 40 7.4 37.4 6.66 249 38 1.02
30 O60218 Aldo-keto reductase family 1 member B10 35 8.5 36.0 7.66 974 45 1.04
31 Q16891 MICOS complex subunit MIC60 85 6.1 83.6 6.08 113 87 1.52
32 P14136 Glial fibrillary acidic protein 162 6.0 49.8 5.42 93 2 2.38
33 O43933 Peroxisome biogenesis factor 1 63 7.6 142.8 5.91 39 2 −2.47
  1. *Log2 Ratio refers to Log2 ratio of spot intensity from FA/MND treatment to No treatment)