Table 2 Protein expression profile by comparison of No treatment and FA/MND treatment of HepG2
Spot No. | Protein ID | Protein name | 2DE | Prediction | Mascot score | Sequence coverage | Log2 Ratio* | ||
|---|---|---|---|---|---|---|---|---|---|
MW(kDa) | pI | MW(kDa) | pI | ||||||
1 | O94925 | Glutaminase kidney isoform, mitochondrial | 57 | 6.9 | 73.4 | 7.85 | 88 | 9 | 1.22 |
6 | P28838 | Cytosol aminopeptidase | 49 | 7.5 | 56.1 | 8.03 | 116 | 9 | 1.05 |
12 | Q562R1 | Beta-actin-like protein 2 | 303 | 4.3 | 40.0 | 5.39 | 33 | 4 | −1.29 |
16 | P00338 | L-lactate dehydrogenase A chain isoform 3 | 33 | 8.8 | 36.6 | 8.44 | 1732 | 80.1 | −1.40 |
19 | P06733 | Alpha-enolase | 47 | 8.5 | 47.1 | 7.01 | 421 | 35 | 1.59 |
20 | Q06830 | Peroxiredoxin-1 | 22 | 8.4 | 22.1 | 8.27 | 545 | 55 | 2.63 |
21 | P16930 | Fumarylacetoacetase | 41 | 7.6 | 46.3 | 6.46 | 118 | 6 | 1.01 |
22 | P68104 | Elongation factor 1-alpha 1 | 50 | 9.8 | 50.1 | 9.1 | 161 | 4 | −2.64 |
23 | P33176 | Kinesin-1 heavy chain | 137 | 6.7 | 109.6 | 6.12 | 136 | 7 | −1.15 |
24 | P43243 | Matrin-3 | 129 | 7.5 | 94.6 | 5.87 | 228 | 19 | − 1.65 |
25 | Q13733 | Sodium/potassium-transporting ATPase subunit alpha-4 | 57 | 6.5 | 114.1 | 6.23 | 28 | 2 | 1.17 |
26 | Q12931 | Heat shock protein 75 kDa, mitochondrial | 79 | 7.3 | 80.1 | 8.3 | 60 | 10 | 1.06 |
27 | P06733 | Alpha-enolase | 48 | 6.8 | 47.1 | 7.01 | 102 | 27 | 1.00 |
28 | P61163 | Alpha-centractin | 43 | 7.3 | 42.6 | 6.19 | 92 | 8 | −1.00 |
29 | Q15365 | Poly(rC)-binding protein 1 | 40 | 7.4 | 37.4 | 6.66 | 249 | 38 | 1.02 |
30 | O60218 | Aldo-keto reductase family 1 member B10 | 35 | 8.5 | 36.0 | 7.66 | 974 | 45 | 1.04 |
31 | Q16891 | MICOS complex subunit MIC60 | 85 | 6.1 | 83.6 | 6.08 | 113 | 87 | 1.52 |
32 | P14136 | Glial fibrillary acidic protein | 162 | 6.0 | 49.8 | 5.42 | 93 | 2 | 2.38 |
33 | O43933 | Peroxisome biogenesis factor 1 | 63 | 7.6 | 142.8 | 5.91 | 39 | 2 | −2.47 |