Fig. 5

NEK10 depleted cells show a decrease in mitochondrial mass. Expression of four different mitochondrial marker proteins (a TOM20, b TFAM, c VDAC and d OXPHOS) was analyzed from HeLa pLKO, HeLa pLKO-sh89 and HeLa pLKO-sh90 total lysates. For immunoblotting, the following antibodies were used: anti-TOMM20, anti-mtTFAM, anti-VDAC1 and anti-OXPHOS. The quantification graphs on the right show averages from 4 independent experiments. The statistical analyses were performed using One-Way ANOVA followed by Tukey’s Multiple Comparison Test. (* = P < 0.05; ** = P < 0.01; *** = P < 0.001)