Fig. 5From: Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches2-DE profiles of (a) untreated medaka and (b) medaka exposed to K. mikimotoi. Proteins were extracted using Trizol method with a clean-up kit. The loading amount of proteins was 100 μg and silver staining was used. Differentially expressed protein spots were circledBack to article page