Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches

Table 4 Identities of differentially expressed proteins extracted from medaka after exposure to K. mikimotoi using Trizol method with a commercial clean-up kit. MASCOT search engine was used for PMF analysis. Protein scores higher than 58 were significant (p ≤ 0.05)

Spot	pI	MW (kDa)	Protein Name	Accession Number	Mascot Score	Sequence Coverage (%)	Fold Change^a
1	4.64	20	Myosin light chain 1, skeletal muscle isoform	gi\|432,932,023	71	50	−2.0
2	4.66	27	14–3-3 protein beta/alpha-1-like	gi\|432,959,056	73	39	−3.0
3	5.23	42	Muscle actin OlMA1	gi\|1,552,222	85	39	−3.7
4	5.23	42	Muscle actin OlMA1	gi\|1,552,222	110	40	−8.6
5	5.23	42	Muscle actin OlMA1	gi\|1,552,222	115	37	− 9.8
6	5.23	42	Muscle actin OlMA1	gi\|1,552,222	79	33	−10.4
7	5.15	58	Keratin, type II cytoskeletal 8-like isoform X1	gi\|432,864,501	109	33	−3.5
8	5.66	30	Apolipoprotein A-I	gi\|327,358,583	117	52	−9.2
9	5.66	30	Apolipoprotein A-I	gi\|327,358,583	85	42	2.2
10	5.97	23	Beta-crystallin A1–1	gi\|432,890,713	83	47	−2.1
11	6.17	47	Beta-enolase	gi\|432,957,740	129	52	−2.8
12	6.17	47	Beta-enolase	gi\|432,957,740	160	58	−3.5
13	6.17	47	Beta-enolase	gi\|432,957,740	119	46	−3.2
14	6.32	42	Creatine kinase M-type	gi\|765,137,894	92	31	−5.2
15	6.32	42	Creatine kinase M-type	gi\|765,137,894	97	31	−9.6
16	6.32	42	Creatine kinase M-type	gi\|765,137,894	99	29	−6.3
17	6.32	42	Creatine kinase M-type	gi\|765,137,894	88	35	−3.1
18	6.09	23	Beta-crystallin A2 isoform X2	gi\|432,964,694	99	55	−3.5
19	6.59	27	Beta-crystallin B1	gi\|432,884,641	92	52	−3.8
20	6.90	26	Triosephosphate isomerase	gi\|432,908,784	79	39	−2.1

ISSN: 1477-5956