Skip to main content

Table 4 Identities of differentially expressed proteins extracted from medaka after exposure to K. mikimotoi using Trizol method with a commercial clean-up kit. MASCOT search engine was used for PMF analysis. Protein scores higher than 58 were significant (p ≤ 0.05)

From: Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches

SpotpIMW (kDa)Protein NameAccession NumberMascot ScoreSequence Coverage (%)Fold Changea
14.6420Myosin light chain 1, skeletal muscle isoformgi|432,932,0237150−2.0
24.662714–3-3 protein beta/alpha-1-likegi|432,959,0567339−3.0
35.2342Muscle actin OlMA1gi|1,552,2228539−3.7
45.2342Muscle actin OlMA1gi|1,552,22211040−8.6
55.2342Muscle actin OlMA1gi|1,552,22211537− 9.8
65.2342Muscle actin OlMA1gi|1,552,2227933−10.4
75.1558Keratin, type II cytoskeletal 8-like isoform X1gi|432,864,50110933−3.5
85.6630Apolipoprotein A-Igi|327,358,58311752−9.2
95.6630Apolipoprotein A-Igi|327,358,58385422.2
105.9723Beta-crystallin A1–1gi|432,890,7138347−2.1
116.1747Beta-enolasegi|432,957,74012952−2.8
126.1747Beta-enolasegi|432,957,74016058−3.5
136.1747Beta-enolasegi|432,957,74011946−3.2
146.3242Creatine kinase M-typegi|765,137,8949231−5.2
156.3242Creatine kinase M-typegi|765,137,8949731−9.6
166.3242Creatine kinase M-typegi|765,137,8949929−6.3
176.3242Creatine kinase M-typegi|765,137,8948835−3.1
186.0923Beta-crystallin A2 isoform X2gi|432,964,6949955−3.5
196.5927Beta-crystallin B1gi|432,884,6419252−3.8
206.9026Triosephosphate isomerasegi|432,908,7847939−2.1
  1. aA negative value denotes down-regulation after treatment