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Fig. 2 | Proteome Science

Fig. 2

From: Identification and validation of serum autoantibodies in children with B-cell acute lymphoblastic leukemia by serological proteome analysis

Fig. 2

Analysis of the autoantibody response in B-ALL using 2-D immunoblotting. 2-D Immunoblotting images showed 6 different immunoreactive protein spots (marked with arrows) specifically recognized by serum from children with B-ALL. Mixed proteins from 3 B-ALL cell were separated by 2-DE and visualized by Coomassie blue staining (a). Mixed proteins were separated by 2-D PAGE and transferred to PVDF membranes. Then, the membranes were incubated with pooled serum from children with B-ALL (b). PVDF membrane incubated with pooled serum from normal controls (c)

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Proteome Science

ISSN: 1477-5956