Fig. 1From: Identification of different proteins binding to Na, K-ATPase α1 in LPS-induced ARDS cell model by proteomic analysisThe workflow of AP-MS (IP-MS) technology. Using this approach with no IP-level antibodies are available against the target protein. Target protein(bait) is co-expressed with affinity tag and forms a complex with the endogenous components, then purified with immobilized tag affinity protein dynabeads and identified with LC–MS/MS after extensively washing off unspecifically bound proteinsBack to article page