Table 3 Amino acid sequence alignment of DLBS1033P—F25 and F85 spots
From: Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus
Band | Identified protein | Amino acid sequence | Sequence coverage (%) |
|---|---|---|---|
D1 | Lumbrokinase | 1 MELPPGTKIV GGIEARPYEF PWQVSVRRKS TDSHFCGGSI INDRWVVCAA 51 HCMQGESPAL VSLVVGEHDS SAASTVRQTH DVDSIFVNEN YNPRTLENDV 101 SVIKTAIAIT FDINVGPICA PDPANDYVYR KSQCSGWGSI NSGGICCPAV 151 LRYVTLNITT NAFCDAVYTS DTIYDDMICA TDNTGMTDRD SCQGDSGGPL 201 SVKDGSGIFS LVGIVSWGIG CASGYPGVYS RVGFHAGWIT DTITNN | 24.81 |
D2 | Lumbrokinase partial | 1 VIGGTDAAPG EFPWQLSQTR GGSHSCGASL LSSNSGLSAS HCVDGALPGS 51 ITVIAGLHDR SGTPGSQEVD ITGYTMHEEY LTGIYTYSND ISILNFATPI 101 TIGGNIQPAT LPADNSNNYL GLTCVISGWG RTSSSNILPD TLQKASIQVI 151 GTDECQTLVD NVLGCRIWDN HICIYDQANS VGSCNGDSGG PLNCPDGTTV 201 VAGITSWGIS SGGDCLQDYP SVYTRTSAYL DWIAANTP | 20.25 |
D3 | Lumbrokinase partial | 1 VIGGTDAAPG EFPWQLSQTR GGSHSCGASL LSSNSGLSAS HCVDGALPGS 51 ITVIAGLHDR SGTPGSQEVD ITGYTMHEEY LTGIYTYSND ISILNFATPI 101 TIGGNIQPAT LPADNSNNYL GLTCVISGWG RTSSSNILPD TLQKASIQVI 151 GTDECQTLVD NVLGCRIWDN HICIYDQANS VGSCNGDSGG PLNCPDGTTV 201 VAGITSWGIS SGGDCLQDYP SVYTRTSAYL DWIAANTP | 20.25 |
D4 | Lumbrokinase | 1 MELPPGTKIV GGIEARPYEF PWQVSVRRKS TDSHFCGGSI INDRWVVCAA 51 HCMQGESPAL VSLVVGEHDS SAASTVRQTH DVDSIFVNEN YNPRTLENDV 101 SVIKTAIAIT FDINVGPICA PDPANDYVYR KSQCSGWGSI NSGGICCPAV 151 LRYVTLNITT NAFCDAVYTS DTIYDDMICA TDNTGMTDRD SCQGDSGGPL 201 SVKDGSGIFS LVGIVSWGIG CASGYPGVYS RVGFHAGWIT DTITNN | 24.81 |
P2 | Annexin | 1 MSTVHEILSK LSLEGDHSLP PSAYATVKAY SNFDADRDAA ALETAIKTKG 51 VDEVTIINIL TNRSNEQRQD IAFAYQRRTK KELSAALKSA LSGHLETVIL 101 GLLKTPAQYD ASELKAAMKG LGTDEDTLIE IICSRTNQEL CEINRVYREM 151 YKTELEKDII SDTSGDFRKL MVALAKGKRC EDTSVIDYEL IDLDARELYD 201 AGVKRKGTDV PKWINIMTER SVPHLQKVFE RYKSYSPYDM LESIKKEVKG 251 DLENAFLNLV QCIQNKQLYF ADRLYDSMKG KGTRDKVLIR IMVSRCEVDM 301 LKIKSEFKRK YGKSLYYFIQ QDTKGDYQRA LLNLCGGED | 7.37 |
P5 | Fibrinolytic protease 1 | 1 MGGEQYIIGG SNASPGEFPW QLSQTRGGSH SCGASLLNAL NGLSASHCVD 51 GAAPGTITVI AGLHDRSGTP GSQEVDITGY TMHENYNQGT NTYANDIAIL 101 HFASAINIGG NVQAALLPAN NNNDYSDLTC VISGWGRTGS SNVLPDTLQK 151 ASIQVIGTTQ CQSLMGSIGN IWDNHICLYD NANNVGSCNG DSGGPLNCPD 201 GGTRVAGVTS WGVSSGAGNC LQTYPSVYTR TSAYLSWIAN NS | 6.20 |
P7 | Lumbrokinase, partial | 1 IGGTDASPGE FPWQLSQTRG GSHSCGASLL NALNGLSASH CVDGAAPGTI 51 TVIAGLHDRS GTPGSQEVDI TGYTMHENYN QGTNTYANDI AILHFASAIN 101 IGGNVQAALL PANNNNDYNG LTCVISGWGR TGSSNVLPDT LQKASIEVIG 151 TTQCQSLMGS IGNIWDNHIC LYDNANNVGS CNGDSGGPLN CPDGGTRVAG 201 VTSWGVSSGA GNCLQTYPSV YTRTSAYLSW IANNS | 19.14 |
P8 | Fibrinolytic protease 1 | 1 MGGEQYIIGG SNASPGEFPW QLSQTRGGSH SCGASLLNAL NGLSASHCVD 51 GAAPGTITVI AGLHDRSGTP GSQEVDITGY TMHENYNQGT NTYANDIAIL 101 HFASAINIGG NVQAALLPAN NNNDYSDLTC VISGWGRTGS SNVLPDTLQK 151 ASIQVIGTTQ CQSLMGSIGN IWDNHICLYD NANNVGSCNG DSGGPLNCPD 201 GGTRVAGVTS WGVSSGAGNC LQTYPSVYTR TSAYLSWIAN NS | 11.57 |
P9 | Fibrinolytic protease 1 | 1 MGGEQYIIGG SNASPGEFPW QLSQTRGGSH SCGASLLNAL NGLSASHCVD 51 GAAPGTITVI AGLHDRSGTP GSQEVDITGY TMHENYNQGT NTYANDIAIL 101 HFASAINIGG NVQAALLPAN NNNDYSDLTC VISGWGRTGS SNVLPDTLQK 151 ASIQVIGTTQ CQSLMGSIGN IWDNHICLYD NANNVGSCNG DSGGPLNCPD 201 GGTRVAGVTS WGVSSGAGNC LQTYPSVYTR TSAYLSWIAN NS | 28.93 |