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Proteomic identification of Drosophila melanogaster male accessory gland proteins, including a pro-cathepsin and a soluble γ-glutamyl transpeptidase



In Drosophila melanogaster, the male seminal fluid contains proteins that are important for reproductive success. Many of these proteins are synthesised by the male accessory glands and are secreted into the accessory gland lumen, where they are stored until required. Previous studies on the identification of Drosophila accessory gland products have largely focused on characterisation of male-specific accessory gland cDNAs from D. melanogaster and, more recently, Drosophila simulans. In the present study, we have used a proteomics approach without any sex bias to identify proteins in D. melanogaster accessory gland secretions.


Thirteen secreted accessory gland proteins, including seven new accessory gland proteins, were identified by 2D-gel electrophoresis combined with mass spectrometry of tryptic fragments. They included protein-folding and stress-response proteins, a hormone, a lipase, a serpin, a cysteine-rich protein and two peptidases, a pro-enzyme form of a cathepsin K-like cysteine peptidase and a γ-glutamyl transpeptidase. Enzymatic studies established that accessory gland secretions contain a cysteine peptidase zymogen that can be activated at low pH. This peptidase may have a role in the processing of female and other male-derived proteins, but is unlikely to be involved in the processing of the sex peptide. γ-Glutamyl transpeptidases are type II integral membrane proteins; however, the identified AG γ-glutamyl transpeptidase (GGT-1) is unusual in that it is predicted to be a soluble secreted protein, a prediction that is supported by biochemical evidence. GGT-1 is possibly involved in maintaining a protective redox environment for sperm. The strong γ-glutamyl transpeptidase activity found in the secretions provides an explanation for the observation that glutamic acid is the most abundant free amino acid in accessory gland secretions of D. melanogaster.


We have applied biochemical approaches, not used previously, to characterise prominent D. melanogaster accessory gland products. Of the thirteen accessory gland secreted proteins reported in this study, six were represented in a D. simulans male accessory gland EST library that was biased for male-specific genes. Therefore, the present study has identified seven new secreted accessory gland proteins, including GGT-1, which was not recognised previously as a secreted accessory gland product.


The male accessory glands (AGs) of Drosophila melanogaster are necessary for male fertility [1, 2]. They are responsible for the synthesis and secretion of a large number of seminal fluid proteins, many of which have not been structurally or functionally characterised. The most studied AG products are male-specific proteins (Acps) that elicit multiple physiological and behavioural effects in post-mated females [3]. These male influences include increased rate of egg-laying, reduced attractiveness to males, rejection of courting males, better sperm storage and a reduction in female life-span [3]. The altered behaviour of mated females can last up to one week and this long-term effect is dependent upon transfer of both sperm as well as seminal fluid. The sex peptide (Acp70A) is partly responsible for eliciting the short-term female responses and solely responsible for the longer lasting changes in post-mated female behaviour [4, 5]. The latter effect is dependent upon the presence of sperm that carry sex peptide bound to the surface of sperm tails [6]. The tethered sex peptide is slowly-released from the sperm by proteolytic cleavage of the N-terminal binding domain from the C-terminal sequence, which contains the signal for the post-mating response [7]. A second AG product (ovulin, ACP26Aa) is also involved in stimulating the early responses in mated females and appears to have a direct effect on ovulation [810]. Ovulin also undergoes proteolysis in the female reproductive tract to release biologically active peptides, a process that is dependent upon another, as yet unidentified, male AG product [11]. Sperm storage in the post-mated female requires Acp36DE, a 122 kDa glycoprotein that is processed to a 68 kDa protein in the female within ten minutes of transfer from the male [1214].

Other seminal fluid proteins that have been studied include peptidase inhibitors, components of the mating plug, lipases and anti-bacterial peptides (reviewed in [3]). An expressed sequence tag screen of a cDNA library prepared from the AGs of D. simulans and purposely enriched for male-specific cDNAs, indicated that there might be in total over 70 Acps in this species [15]. Several classes of enzymes, including lipases and peptidases, were well represented in the D. simulans AG ESTs, as were peptidase inhibitors and immune defence proteins [16]. This information has led to a re-assessment of the total number of secreted D. melanogaster Acps, which is now thought to be 52 [17]. However, this number will exclude AG products, which are highly expressed in female tissues, but are vital for male reproductive success. Specific antibodies raised to thirteen D. melanogaster Acps showed that these male AG proteins have multiple fates once transferred to the female reproductive tract, with some proteins entering the female haemolymph [18].

We now report the direct characterisation of protein products of D. melanogaster AGs, which were resolved by 2D-gel electrophoresis and identified by mass spectrometry of tryptic peptides. This study identifies thirteen AG proteins, including protein-folding and stress-response proteins, a lipase, a cysteine-rich protein and two peptidases, a pro-form of a cathepsin-like cysteine peptidase and a soluble γ-glutamyl transpeptidase. Seven of these proteins have not previously been identified as secreted AG products. We also show that the AG secretions possess cysteine peptidase and γ-glutamyl transpeptidase activities and that the cysteine peptidase is synthesised primarily as an inactive zymogen. The cathepsin K-like cysteine peptidase is a candidate for the processing enzyme responsible for the delayed cleavage of male proteins and peptides in the uterus of the post-mated female.

Results and discussion

Identification of secreted AG proteins

AG contents were analysed by 2D-gel electrophoresis; the first dimension used either pH 3–10 or pH 4–7 IPG strips (Fig. 1). The gels were Coomassie stained and the most intense resolved protein spots subjected to trypsin digestion and peptide fragment analysis by either MALDI-MS or LC-MS/MS. Thirteen AG proteins with predicted signal peptides for secretion were identified using this method and their functions were inferred from comparison of the primary structure to members of protein families with known biological activity (Table 1). Almost half of these proteins could be classed as stress response proteins or as proteins required for the efficient and correct folding of newly synthesised proteins in the ER lumen. The other group of proteins contains two peptidases, a lipase, a protein hormone (Acp36DE), a cysteine-rich protein and a serpin.

Figure 1
figure 1

A representative 2-D gel (pH 4–7) stained with Coomassie blue, showing the separation of proteins from 80 male AGs. Protein spots that were unambiguously identified as secreted proteins in this experiment are labelled. 1, CG9429; 2, CG4147; 3, CG4847; 4, CG17575.

Table 1 D. melanogaster accessory gland proteins identified by proteomics. AG proteins with a secretory signal peptide identified by either MALDI-MS or LC-MS/MS of tryptic fragments of AG protein spots excised from 2D gels. Data presented in parenthesis is derived from MALDI-MS analysis

Protein folding and stress response proteins

CG2852 (cyclophilin-like) and CG9847 (Fkbp-13) are peptidyl-prolyl cis-trans isomerases that catalyse the cis-trans isomerisation of peptidyl-prolyl bonds in polypeptide chains. They belong to two different structural classes that are phylogenetically conserved and are known to have multiple roles in the folding, assembly and trafficking of cellular proteins [19]. Although considered to be primarily intracellular proteins, some mammalian cells secrete cyclophilins into the extracellular environment, where they have an important role in mediating cellular responses to oxidative stress and in modulating immunity. CG6988 is a protein disulfide isomerase (PDI) that contains two thioredoxin-like folds and is highly expressed at all stages of development [20]. The PDI family of proteins catalyse the formation, reduction and isomerisation of disulphide bonds and can protect cells from hypoxic cell death [21]. Their ability to bind polypeptide chains allows PDI to act as a molecular chaperone assisting with the folding of polypeptides and increasing the yield of correctly folded proteins.

Four other molecular chaperones (CG9429/calreticulin; CG4147/HSP70-3; CG2918; CG5520/Gp93) were identified in this proteomic study of male AGs. D. melanogaster calreticulin belongs to the highly conserved family of lectin-like Ca2+-binding ER proteins that have diverse cellular roles in mammals, including regulation of intracellular Ca2+, the correct folding of glycoproteins, integrin-mediated Ca2+ signalling, phagocytosis and cell adhesion [22, 23]. HSP70-3 is a BiP homologue involved in assembling protein complexes inside the endoplasmic reticulum [24]. Gp93 is a highly conserved heat shock protein (HSP) of the vertebrate gp96 family of endoplasmic reticulum proteins. Galewsky et al. have reported that Gp93 accumulates at cell borders suggesting that the D. melanogaster protein can be exported from the cell to the extracellular surface [25]. CG2918 is structurally also a member of the HSP family of proteins with strong homology to mammalian hypoxia up-regulated protein ROP150 [26].

Five of the aforementioned AG protein-folding and stress-response proteins possess a K/HDEL C-terminal sequence (the C-terminus of CG2918 is HSEL) suggesting that these proteins are resident in the lumen of the endoplasmic reticulum. However, it is well documented that proteins entering the secretory pathway can escape KDEL-mediated endoplasmic reticulum-retention to have a functional role at the cell surface or in the extracellular milieu [23, 27]. Indeed, recent studies have shown that cell-surface chaperone proteins can have important roles in reproduction by functioning as intercellular signalling molecules that facilitate sperm-oocyte interaction [2830].

Acp36DE (CG7157), cysteine-rich protein (CG17575) and serpin-3 (spn-3, CG9334)

Acp36DE (CG7157) has been extensively studied and shown to be required for sperm storage [12, 14, 31], although the mechanism by which this is achieved is not known. CG17575 has been shown by comparative structural modelling to be a member of the CRISP family (cysteine-rich secretory protein) [16]. Interestingly, mammalian CRISP-1 is secreted by the proximal epididymis, binds to the sperm surface, and appears to be involved in gamete fusion [32]. CRISP-2 is secreted from spermatocytes and facilitates enhanced binding of spermatocytes to Sertoli cells. Allurin, another CRISP, acts as a sperm chemoattractant produced by female Xenopus as part of the egg jelly [33]. In addition to these suggested roles in reproduction, CRISP proteins of the CRISP-3 class might also form part of the innate immune defence system [34]. Antibodies raised to recombinant CG17575 were used in a recent study to determine the fate of the protein in the mated female. Western blot analyses suggested a broad distribution for CG17575 in female reproductive tissues, including the uterus, oviduct and egg surface [18], consistent with a possible role in gamete fusion.

Two members (Acp76A and Acp62F) of the serpin family were previously known to be present in D. melanogaster AG secretions. The proteomic identification of SPN-3 takes the number of D. melanogaster AG serpins to three and this number might be an underestimate, since Swanson and colleagues identified six AG serpin genes in D. simulans, including the orthologue of Spn-3 [15]. The expression of a number of serpin family genes in the AG probably reflects the importance to the male of controlling proteolysis by serine peptidases in the seminal fluid [16]. Evidence for such a role for serpins in animal reproduction comes from studies on male mice lacking the serpins, PCI and nexin-1 [35, 36]. These animals display abnormal spermatogenesis and malformation of the copulatory plug presumably as a result of a failure to control peptidase activity. Immunological studies on the fates of D. melanogaster Acps in the female, post-copulation, identified CG9334 in both the mating plug and the sperm storage organs [18].

Acid lipase (CG31872)

The AGs of D. melanogaster are known to be a rich source of an acid lipase capable of cleaving triacylglycerol and to some extent cholesterol esters. Smith et al. showed that the lipase activity of males was halved after mating, whereas the activity in mated females was three-times that of virgin female flies, indicating transfer in the seminal fluid to the female during copulation [37]. Our proteomics analysis has identified CG31872 as a candidate for this activity. These enzymes might have an important role in providing energy for motile sperm by hydrolysing triglycerides to release free fatty acids for β-oxidation. The work of Mueller et al. [17]identified five acid lipase related AG ESTs of D. simulans and therefore we might expect additional lipases to be present in the AGs of D. melanogaster.

Procathepsin protein (CG4847) and the demonstration of pro-form of a cysteine peptidase in AG secretions

CG4847 encodes a protein that structurally belongs to the papain (C1A) family of cysteine peptidases and is related to mammalian cathepsin K, L and S (Fig. 2) [38]. CG4847 has a predicted molecular weight of 44 kDa in its unprocessed form and, uniquely for this peptidase family, posesses a Gly-Gly-Gly-Phe/Leu repeat sequence close to the N-terminus of the protein. BLAST search of the predicted proteins of the Drosophila pseudoobscura genome identified GA18475 as a close homologue of CG4847 that shares 68% identity at the amino acid level. The predicted D. pseudoobscura also has a Gly/Leu-rich region following a signal peptide; however this region is shorter than that in CG4847 (Fig. 2).

Figure 2
figure 2

ClustalX amino acid sequence alignment of D. melanogaster cathepsin K (Dm cathepsinK, CG4847), D. pseudoobscura cathepsin K (Dp cathepsinK, GA18475) and human cathepsin K, S, and L. Active site residues, stars; Gly-rich region, dashed line; predicted cleavage to generate mature cathepsin, filled block arrow; tryptic peptides identified by mass spectrometry, underline. Conserved amino acids are in black.

Cathepsins are typically synthesised as inactive pro-enzymes that, under favourable conditions, undergo intracellular auto-cleavage that removes an N-terminal pro-domain to generate an active peptidase of around 24 kDa in size [39]. The observation that CG4847 migrates in SDS-polyacrylamide gel electrophoresis with a mass of around 50 kDa and the fact that the peptide mass fingerprint contained five peptides from the predicted pro-domain, indicates that the enzyme is secreted with a typical zymogen structure (Fig. 2). We sought enzymatic evidence for the synthesis and secretion of a pro-cathepsin cysteine peptidase by male AGs. AG contents were incubated in activation buffer, prior to performing a standard assay for C1A cathepsins using the substrate Cbz-Leu-Arg-MCA in a continuous assay for peptidase activity. The hydrolysis of the substrate was linear with time and was strongly inhibited by two irreversible inhibitors of cysteine peptidases (E64 and K11777), all at a concentration of 10 μM (Table 2). Mammalian pro-cathepsin K, like many other cysteine peptidases, undergoes autoactivation as a result of a conformational change on lowering the pH [40]. The induced structural change exposes the pro-cathepsin K active site to increase the susceptibility of the pro-peptide to cleavage. When the activation procedure for the AG cysteine peptidase was performed at 4°C rather than at 35°C, the peptidase activity was greatly reduced (Table 2). This result is consistent with the peptidase being secreted as a zymogen that can be activated in a temperature-dependent manner at low pH. It is possible that the activation of the AG cysteine peptidase occurs after insemination by autocatalysis after experiencing a change in pH in the female reproductive tract or by exposure to another peptidase either present in the seminal fluid or secreted from female reproductive tissues.

Table 2 Peptidase activity of D. melanogaster accessory glands. Results are expressed as the mean of three experiments ± s.e.m

A potential role for the CG4847 is in the proteolytic processing of hormone precursors. The predicted protein is structurally related to mammalian cathepsin L, which in secretory vesicles of chromaffin cells functions as a prohormone processing peptidase responsible for the production of enkephalin peptides [41]. The cathepsin L recognises monobasic and dibasic processing sites with the positively charged side-chains occupying either the S1 or S1' sub-sites. Two D. melanogaster AG prohormones, ovulin and sex peptide, undergo proteolytic processing in the post-mated female and in both cases, basic residues are the cleavage recognition sites [7, 11]. CG4847 might perform a similar prohormone processing role to cathepsin L and be involved in the cleavage of both ovulin and sex peptide after transfer to the mated female. We have investigated the possibility that the AG cysteine peptidase is responsible for the cleavage of the sex peptide by testing whether the activated enzyme can cleave sex peptide 1–13 (WEWPWNRKPTKFP), which contains two potential prohormone cleavage sites, at the Arg7-Lys8 and Lys11-Phe12 peptide bonds. HPLC analysis with UV detection (280 nm) identified a single major cleavage product that was identified as WEWPWNRKPTK by MALDI-MS ([M+H]+ m/z 1528). This hydrolysis was completely blocked in the presence of 10 μM E64, confirming that the AG cysteine peptidase activity was responsible. Peng et al. have shown that the N-terminal region of sex peptide is required for sperm attachment and for the long-term post-mating response of females [7]. They have also shown that mutating the Arg7 and Lys8 to Gln7 and Gln8 prevents the release of sex peptide from the surface of the sperm tail over a 5 day period, which suggests that proteolysis occurs at, or close to, the dibasic motif. Since the AG cysteine peptidase activity cleaves Lys11-Phe12, which is four peptide bonds away from Arg7-Lys8, it is unlikely that the enzyme has a major role in the processing of the sex peptide in the post-mated female.

γ-Glutamyl transpeptidase (CG6461, GGT-1) and detection of γ-glutamyl transpeptidase activity in AG secretions

CG6461(GGT-1) is predicted to be a 62 kDa γ-glutamyl transpeptidase (also known as γ-glutamyl transferase) and a member of the T3 family of threonine peptidases [42]. These enzymes are abundant on the luminal surface of secretory and absorptive epithelial cells and are responsible for the transfer of γ-glutamate from glutathione (GSH), glutathione conjugates and glutathione disulphide (GSSG) to an acceptor molecule, which may be another peptide, an amino acid, or water. The proenzyme undergoes autocatalytic cleavage to generate the active γ-glutamyl transpeptidase, comprising two subunits of around 40 kDa and 20 kDa [43]. The catalytic nucleophile is the hydroxyl group of the threonine found at the N-terminus of the smaller processed subunit [44]. The AG peptide fragments identified in the present study came from a protein spot with an estimated size of around 20 kDa and corresponded to sequences within the predicted smaller subunit of GGT-1 (Fig. 3). The SignalP 3.0 server predicts with high probability that GGT-1 is a preproprotein comprising an N-terminal signal peptide for secretion and that this is cleaved at the Gly30-Leu31 bond to generate a soluble, secreted γ-glutamyl transpeptidase [45]. The predicted soluble nature of GGT-1 is unusual for members of this family of enzymes, since eukaryotic γ-glutamyl transpeptidases are typically type II integral membrane proteins found on the external surfaces of cells [42]. There are three other γ-glutamyl transpeptidase genes (CG17636, CG1492 and CG4829) in the D. melanogaster genome, all of which are predicted by SignalP 3.0 to encode proteins that conform to a membrane-bound structure. In D. pseudoobscura, γ-glutamyl transpeptidase genes are fewer in number and the family is represented by one secreted (GA9613) and only one membrane-bound γ-glutamyl transpeptidase (GA13353): these are closely related to D. melanogaster GGT-1 and CG4829, respectively.

Figure 3
figure 3

ClustalX alignment of D. melanogaster GGT-1 (CG6461) and human GGT-1. Hydrophobic signal sequence/transmembrane anchor, dashed line; predicted cleavage of the signal peptide for GGT-1, filled block arrow; predicted cleavage site necessary for activation, open block arrow ; active site Thr, *; tryptic peptides identified by mass spectrometry, underline. Conserved amino acids are in black.

We found strong γ-glutamyl transpeptidase activity (28.1 ± 1.7 μmoles of p-nitroanilide released/h/AG; mean ± standard error of the mean, n = 3) in the Drosophila AG using γ-glutamyl-p-nitroanilide as the substrate donor and the dipeptide, Gly-Gly, as the acceptor. There was a linear increase in enzyme activity, which was absolutely dependent on the presence of Gly-Gly and was inhibited by acivicin (0.4 mM, 98% inhibition), a selective inhibitor of γ-glutamyl transpeptidase. The AG γ-glutamyl transpeptidase activity is not associated with cell membranes since almost all of the activity of the AG homogenate was found in the supernatant after centrifugation at 60,000 g for 2 h (25.1 ± 2.3 μmoles of p-nitroanilide released/h/AG; mean ± standard error of the mean, n = 3).

The identification of a secreted γ-glutamyl transpeptidase in the AG is of particular interest in respect of redox control during reproduction. γ-Glutamyl transpeptidase has a central position in the 'γ-glutamyl cycle', generating glutamate and Cys-Gly by cleavage of GSH. The dipeptide is subsequently cleaved to its constituent amino acids [46], which are taken-up across the plasma membrane and used in the re-synthesis of intracellular GSH. GSH provides reducing equivalents for the reduction of hydrogen peroxide and lipid hydroperoxides by glutathione peroxidases and peroxiredoxins, and is required for detoxification reactions carried out by glutathione S-transferases [47, 48]. The maintenance of a high intracellular concentration of GSH is therefore important for preventing injury to the germ cells by reactive oxygen species and xenobiotics, and is highly dependent upon the breakdown and recycling of extracellular GSH compounds [48].

The soluble AG γ-glutamyl transpeptidase activity provides an explanation for the relatively high levels of free glutamic acid known to be present in AG secretions of virgin males of D. melanogaster and the rise in uterine glutamate in the post-mated female [49].


Interest in the male AG products of D. melanogaster has mainly focused on the proteins and peptides that are responsible for altering the behaviour and physiology of the post-mated female. There have been few biochemical studies of AG enzymes that are secreted into the seminal fluid for transfer to the female, although their importance for reproduction is recognised. The sequencing of male-specific cDNAs of D. simulans AG gave an indication of the variety of new proteins that might be present in the seminal fluid of this species and probably other drosophilids. However, this study was biased against proteins that are expressed in both adult male and female flies, and did not shed light on any post-translational modifications that might be critical for biological activity. We have used the complementary approach of proteomics to expand our knowledge of the secreted AG proteins, mainly enzymes and stress-response proteins, of D. melanogaster. We have provided enyzmatic evidence for an inactive cysteine protease and a processed and fully active γ-glutamyl transpeptidase in support of the proteomics data. The cysteine peptidase might undergo activation in the extracellular environment and, if transferred to the female during copulation, might have a role in the processing of male derived proteins (e.g. ovulin) in the mated female. D. melanogaster is amenable to reverse genetic approaches, which can now be used to determine the importance and precise role of the identified AG proteins in reproduction.



Peptidase substrates and inhibitors, unless otherwise stated, were purchased from Sigma-Aldrich Co. Ltd., Poole, U.K. K11777 (N-methylpiperazine-urea-phenylalanine-homophenylalanine-vinylsulfone-benzene) was a gift from the Sandler Center for Basic Research in Parasitic Diseases, Department of Pathology, University of California San Francisco.


Oregon R D. melanogaster were maintained on oatmeal/molasses/agar medium at 25°C with a 12:12 h light/dark regime [50]. For the collection of AG contents for proteomic and enzymatic analysis, males (3–5 days post-eclosion) were separated from females at least 24 h prior to dissection.


AGs (80 pairs) were excised in ice-cold Drosophila Ringer solution [51], or 5 mM Tris-HCl (pH 7.5), containing peptidase inhibitors (complete, Mini, EDTA-free, Roche Diagnostics Ltd, Bell Lane, Lewes, East Sussex BN7 1LG). The AGs were then centrifuged at 10 000 g at 4°C to separate AG contents from the tissue. The supernatant was then removed and stored at -20°C until required. Protein samples were processed using a "2D clean-up kit" (Amersham Biosciences UK Limited, Pollards Wood, Nightingales Lane, Chalfont St Giles, Buckinghamshire). AG contents were solubilised in rehydration buffer (8 M urea, 4% (w/v) CHAPS, 50 mM dithiothreitol, 0.2% (v/v) "Bio-Lyte" Ampholytes, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA) prior to soaking of either pH 3–10 or pH 4–7 11 cm IPG strip (Bio-Rad). Isoelectric focusing was performed using a PROTEAN IEF cell (Bio-Rad) for 40000 Vh. The strip was then equilibrated in two base buffers (50 mM Tris/Cl, 2% (v/v) SDS, 6 M urea, and 20% glycerol) containing first 1.4 M diothiothreitol and then 1.3 M iodoacetamide. The second dimension used precast 8–16% Tris/HCl gels (Bio-Rad) and was run for 70 min at 200 V. The gel was washed three times for 15 min prior to staining with Coomassie ("biosafe" Bio-Rad) for 3 h before de-staining with water.

Stained spots of protein were cut using a Proteome Works spot-cutter (Bio-Rad) before being placed in 50 mM ammonium bicarbonate and 50 % (v/v) acetonitrile. The gel piece was sonicated for 10 min with the supernatant being removed and discarded. This was repeated before acetonitrile was added and then the gel was dried. The trypsin digest was then carried out by adding 5 μl trypsin solution (2 ng of trypsin in 25 mM ammonium bicarbonate) to the gel piece and allowing it to be absorbed before addition of another 5 μl of 25 mM ammonium bicarbonate 30 min later. Digestion was allowed to proceed for 16 h at 37°C.

MALDI-MS was performed using a matrix of 5 mg of α-cyano-4-hydroxy-cinnamic acid in 1 ml ethanol/acetonitrile (1:1, v/v) with the peptide ACTH used as an internal standard. The matrix was then mixed with the sample in a ratio of 1:1 with 1 μl being spotted onto a MALDI target plate for analysis using MALDI-TOF (Micromass M@LDI L/R system) to generate a peptide mass fingerprint. The NCBI and MSDB peptide databases were searched using Mascot with, 100 p.p.m. accuracy and carbamidomethyl transformation selected. Some protein spots were analysed by LC-MS/MS and the fragmentation data was used to search the NCBI and MSDB peptide databases using Mascot as described previously [52]

Enzyme assays

AG contents were separated from the AG tissue by centrifugation in small volumes of Ringer solution, typically 20 pairs of AGs in 40 μl, at 10 000 g at 4°C. For the determination of cysteine peptidase activity, AG samples were incubated in activation buffer (100 mM sodium acetate buffer, pH 5.5; 5 mM EDTA; 20 mM cysteine) at 35°C, either without inhibitor or with one of two cysteine peptidase inhibitors (E64 and K11777). After 1 h, the substrate (Cbz-Leu-Arg-MCA) was added to a final concentration of 10 μM in 1% (v/v) dimethyl sulfoxide and the fluorescence emitted during the reaction was measured every 90 seconds in black 96-well microtitre plates (Corning Life Sciences, High Wycombe, U.K.) at ambient temperature using a Victor2 fluorometer (PerkinElmer™, Finland) and an excitation λ of 380 nm and emission λ of 460 nm. Reaction rates were linear up to 2 h (1 unit of activity is 1 pmol of 7-AMC released/h). Cysteine peptidase activity towards sex peptide1-13 was performed by incubating the contents from 50 AGs in 50 μl of activation buffer for 1 h at 35°C, before adding sex peptide1-13 (50 μM, final concentration). After incubation for 12 h at 35°C, hydrolytic activity was terminated by lowering the pH to 2 with 5 μl of 8% (v/v) TFA. Cleavage products were analysed by reversed phase HPLC using a Phenomenex Jupiter C18 column (5 μm particles, 250 × 4.6 mm; Phenomenex, Macclesfield, U.K.) and an elution gradient of 12% acetonitrile rising to 60% acetonitrile in 0.1% TFA over 25 min, at a flow rate of 1 ml/min. The elution of tryptophan containing peptides was monitored at 280 nm. The major product peak was collected and analysed by MALDI-TOF MS.

γ- Glutamyl transpeptidase activity was assayed using γ-glutamyl-p-nitroanilide (1 mM) as the γ-glutamyl donor and the dipeptide Gly-Gly (20 mM) as the acceptor in Tris-HCl buffer (0.1 M, pH 8) [42]. The release of p-nitroaniline was monitored every 10 sec in 96-well plastic plates (NuncTM, Denmark) using a SPECTRA MAX 340PC spectrometer (Molecular Devices, Wokingham, U.K.) set at 410 nm (ε = 8800 M-1. cm-1) and a temperature of 25°C. The reaction rate was linear up to 1 h (1 unit of activity is 1 nmol of p-nitroaniline released/h). The soluble nature of the γ- glutamyl transpeptidase was established by homogenising ten AGs in 200 μl of Ringer's solution in a glass homogeniser (GPE Ltd., Leighton Buzzard, UK) and centrifuging the homogenate at 60,000 g for 2 h in a TLA110 Beckman rotor using an Optima Max Ultracentrifuge (Beckman Coulter UK Ltd, High Wycombe, Buckinghamshire, U.K.). The enzyme activity of resulting soluble fraction was compared to the activity found in a sample of the homogenate taken prior to centrifugation.


  1. Chen PS: The accessory gland proteins in male Drosophila : structural, reproductive, and evolutionary aspects. Experientia 1996, 52: 503–510. 10.1007/BF01969718

    Article  CAS  PubMed  Google Scholar 

  2. Wolfner MF: Tokens of love: functions and regulation of Drosophila male accessory gland products. Insect Biochem Mol Biol 1997, 27: 179–192. 10.1016/S0965-1748(96)00084-7

    Article  CAS  PubMed  Google Scholar 

  3. Chapman T, Davies SJ: Functions and analysis of the seminal fluid proteins of male Drosophila melanogaster fruit flies. Peptides 2004, 25: 1477–1490. 10.1016/j.peptides.2003.10.023

    Article  CAS  PubMed  Google Scholar 

  4. Kubli E: Sex-peptides: seminal peptides of the Drosophila male. Cell Mol Life Sci 2003, 60: 1689–1704. 10.1007/s00018-003-3052

    Article  CAS  PubMed  Google Scholar 

  5. Chapman T, Bangham J, Vinti G, Seifried B, Lung O, Wolfner MF, Smith HK, Partridge L: The sex peptide of Drosophila melanogaster : female post-mating responses analyzed by using RNA interference. Proc Natl Acad Sci USA 2003, 100: 9923–9928. 10.1073/pnas.1631635100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Liu H, Kubli E: Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster . Proc Natl Acad Sci USA 2003, 100: 9929–9933. 10.1073/pnas.1631700100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Peng J, Chen S, Busser S, Liu H, Honegger T, Kubli E: Gradual release of sperm bound sex-peptide controls female postmating behavior in Drosophila . Curr Biol 2005, 15: 207–213. 10.1016/j.cub.2005.01.034

    Article  CAS  PubMed  Google Scholar 

  8. Heifetz Y, Lung O, Frongillo EA Jr, Wolfner MF: The Drosophila seminal fluid protein Acp26Aa stimulates release of oocytes by the ovary. Curr Biol 2000, 10: 99–102. 10.1016/S0960-9822(00)00288-8

    Article  CAS  PubMed  Google Scholar 

  9. Chapman T, Herndon LA, Heifetz Y, Partridge L, Wolfner MF: The Acp26Aa seminal fluid protein is a modulator of early egg hatchability in Drosophila melanogaster . Proc Biol Sci 2001, 268: 1647–1654. 10.1098/rspb.2001.1684

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Herndon LA, Wolfner MF: A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating. Proc Natl Acad Sci USA 1995, 92: 10114–10118.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Heifetz Y, Vandenberg LN, Cohn HI, Wolfner MF: Two cleavage products of the Drosophila accessory gland protein ovulin can independently induce ovulation. Proc Natl Acad Sci USA 2005, 102: 743–748. 10.1073/pnas.0407692102

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Bertram MJ, Neubaum DM, Wolfner MF: Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage. Insect Biochem Mol Biol 1996, 26: 971–980. 10.1016/S0965-1748(96)00064-1

    Article  CAS  PubMed  Google Scholar 

  13. Neubaum DM, Wolfner MF: Mated Drosophila melanogaster females require a seminal fluid protein, Acp36DE, to store sperm efficiently. Genetics 1999, 153: 845–857.

    PubMed Central  CAS  PubMed  Google Scholar 

  14. Bloch Qazi MC, Wolfner MF: An early role for the Drosophila melanogaster male seminal protein Acp36DE in female sperm storage. J Exp Biol 2003, 206: 3521–3528. 10.1242/jeb.00585

    Article  PubMed  Google Scholar 

  15. Swanson WJ, Clark AG, Waldrip-Dail HM, Wolfner MF, Aquadro CF: Evolutionary EST analysis identifies rapidly evolving male reproductive proteins in Drosophila . Proc Natl Acad Sci USA 2001, 98: 7375–7379. 10.1073/pnas.131568198

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Mueller JL, Ripoll DR, Aquadro CF, Wolfner MF: Comparative structural modeling and inference of conserved protein classes in Drosophila seminal fluid. Proc Natl Acad Sci U SA 2004, 101: 13542–13547. 10.1073/pnas.0405579101

    Article  CAS  Google Scholar 

  17. Mueller JL, Ravi Ram K, McGraw LA, Bloch Qazi MC, Siggia ED, Clark AG, Aquadro CF, Wolfner MF: Cross-species comparison of Drosophila male accessory gland protein genes. Genetics 2005, 171: 131–143. 10.1534/genetics.105.043844

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Ravi Ram K, Ji S, Wolfner MF: Fates and targets of male accessory gland proteins in mated female Drosophila melanogaster . Insect Biochem Mol Biol 2005, 35: 1059–1071. 10.1016/j.ibmb.2005.05.001

    Article  CAS  PubMed  Google Scholar 

  19. Galat A: Peptidylproline cis-trans-isomerases: immunophilins. Eur J Biochem 1993, 216: 689–707. 10.1111/j.1432-1033.1993.tb18189.x

    Article  CAS  PubMed  Google Scholar 

  20. McKay RR, Zhu L, Shortridge RD: A Drosophila gene that encodes a member of the protein disulfide isomerase/phospholipase C-alpha family. Insect Biochem Mol Biol 1995, 25: 647–654. 10.1016/0965-1748(95)00001-C

    Article  CAS  PubMed  Google Scholar 

  21. Ellgaard L, Ruddock LW: The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep 2005, 6: 28–32. 10.1038/sj.embor.7400311

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Michalak M, Corbett EF, Mesaeli N, Nakamura K, Opas M: Calreticulin: one protein, one gene, many functions. Biochem J 1999, 344: 281–292. 10.1042/0264-6021:3440281

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Johnson S, Michalak M, Opas M, Eggleton P: The ins and outs of calreticulin: from the ER lumen to the extracellular space. Trends Cell Biol 2001, 11: 122–129. 10.1016/S0962-8924(01)01926-2

    Article  CAS  PubMed  Google Scholar 

  24. Kirkpatrick RB, Ganguly S, Angelichio M, Griego S, Shatzman A, Silverman C, Rosenberg M: Heavy chain dimers as well as complete antibodies are efficiently formed and secreted from Drosophila via a BiP-mediated pathway. J Biol Chem 1995, 270: 19800–19805. 10.1074/jbc.270.34.19800

    Article  CAS  PubMed  Google Scholar 

  25. Drysdale RA, Crosby MA: FlyBase: genes and gene models. Nucleic Acids Res 2005, 33: D390–395. 10.1093/nar/gki046

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Bando Y, Ogawa S, Yamauchi A, Kuwabara K, Ozawa K, Hori O, Yanagi H, Tamatani M, Tohyama M: 150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells. Am J Physiol Cell Physiol 2000, 278: C1172–1182.

    CAS  PubMed  Google Scholar 

  27. Turano C, Coppari S, Altieri F, Ferraro A: Proteins of the PDI family: unpredicted non-ER locations and functions. J Cell Physiol 2002, 193: 154–163. 10.1002/jcp.10172

    Article  CAS  PubMed  Google Scholar 

  28. Calvert ME, Digilio LC, Herr JC, Coonrod SA: Oolemmal proteomics – identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol 2003, 1: 27. 10.1186/1477-7827-1-27

    Article  PubMed Central  PubMed  Google Scholar 

  29. Tutuncu L, Stein P, Ord TS, Jorgez CJ, Williams CJ: Calreticulin on the mouse egg surface mediates transmembrane signaling linked to cell cycle resumption. Dev Biol 2004, 270: 246–260. 10.1016/j.ydbio.2004.02.008

    Article  CAS  PubMed  Google Scholar 

  30. Asquith KL, Harman AJ, McLaughlin EA, Nixon B, Aitken RJ: Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction. Biol Reprod 2005, 72: 328–337. 10.1095/biolreprod.104.034470

    Article  CAS  PubMed  Google Scholar 

  31. Chapman T, Neubaum DM, Wolfner MF, Partridge L: The role of male accessory gland protein Acp36DE in sperm competition in Drosophila melanogaster . Proc Biol Sci 2000, 267: 1097–1105. 10.1098/rspb.2000.1114

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Cuasnicu PS, Ellerman DA, Cohen DJ, Busso D, Morgenfeld MM, Da Ros VG: Molecular mechanisms involved in mammalian gamete fusion. Arch Med Res 2001, 32: 614–618. 10.1016/S0188-4409(01)00321-6

    Article  CAS  PubMed  Google Scholar 

  33. Olson JH, Xiang X, Ziegert T, Kittelson A, Rawls A, Bieber AL, Chandler DE: Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins. Proc Natl Acad Sci USA 2001, 98: 11205–11210. 10.1073/pnas.211316798

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  34. Kratzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD: The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3. Eur J Biochem 1996, 236: 827–836. 10.1111/j.1432-1033.1996.t01-1-00827.x

    Article  CAS  PubMed  Google Scholar 

  35. Uhrin P, Dewerchin M, Hilpert M, Chrenek P, Schofer C, Zechmeister-Machhart M, Kronke G, Vales A, Carmeliet P, Binder BR, Geiger M: Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility. J Clin Invest 2000, 106: 1531–1539.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Murer V, Spetz JF, Hengst U, Altrogge LM, de Agostini A, Monard D: Male fertility defects in mice lacking the serine protease inhibitor protease nexin-1. Proc Natl Acad Sci USA 2001, 98: 3029–3033. 10.1073/pnas.051630698

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  37. Smith GM, Rothwell K, Wood SL, Yeaman SJ, Bownes M: Specificity and localization of lipolytic activity in adult Drosophila melanogaster . Biochem J 1994, 304: 775–779.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  38. Bromme D: Cathepsin K. In Handbook of Proteolytic Enzymes. Volume 2. Edited by: Barrett AJ, Rawlings ND, Woessner JF. London: Elsevier Academic Press; 2004:1092–1097.

    Google Scholar 

  39. Turk B, Turk D, Turk V: Lysosomal cysteine proteases: more than scavengers. Biochim Biophys Acta 2000, 1477: 98–111.

    Article  CAS  PubMed  Google Scholar 

  40. McQueney MS, Amegadzie BY, D'Alessio K, Hanning CR, McLaughlin MM, McNulty D, Carr SA, Ijames C, Kurdyla J, Jones CS: Autocatalytic activation of human cathepsin K. J Biol Chem 1997, 272: 13955–13960. 10.1074/jbc.272.21.13955

    Article  CAS  PubMed  Google Scholar 

  41. Hook V, Yasothornsrikul S, Greenbaum D, Medzihradszky KF, Troutner K, Toneff T, Bundey R, Logrinova A, Reinheckel T, Peters C, Bogyo M: Cathepsin L and Arg/Lys aminopeptidase: a distinct prohormone processing pathway for the biosynthesis of peptide neurotransmitters and hormones. Biol Chem 2004, 385: 473–480. 10.1515/BC.2004.055

    Article  CAS  PubMed  Google Scholar 

  42. Hiratake J, Suzuki H, Kumagai H: gamma-Glutamyl transpeptidase and its precursor. In Handbook of Proteolytic Enzymes. Volume 2. Edited by: Barrett AJ, Rawlings ND, Woessner JF. London: Elsevier Academic Press; 2004:2090–2094.

    Google Scholar 

  43. Suzuki H, Kumagai H: Autocatalytic processing of gamma-glutamyltranspeptidase. J Biol Chem 2002, 277: 43536–43543. 10.1074/jbc.M207680200

    Article  CAS  PubMed  Google Scholar 

  44. Inoue M, Hiratake J, Suzuki H, Kumagai H, Sakata K: Identification of catalytic nucleophile of Escherichia coli gamma-glutamyltranspeptidase by gamma-monofluorophosphono derivative of glutamic acid: N-terminal thr-391 in small subunit is the nucleophile. Biochemistry 2000, 39: 7764–7771. 10.1021/bi000220p

    Article  CAS  PubMed  Google Scholar 

  45. Bendtsen JD, Nielsen H, von Heijne G, Brunak S: Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 2004, 340: 783–795. 10.1016/j.jmb.2004.05.028

    Article  PubMed  Google Scholar 

  46. Hanigan MH: gamma-Glutamyl transpeptidase, a glutathionase: its expression and function in carcinogenesis. Chem Biol Interact 1998, 111–112: 333–342. 10.1016/S0009-2797(97)00170-1

    Article  PubMed  Google Scholar 

  47. Baker MA, Aitken RJ: The importance of redox regulated pathways in sperm cell biology. Mol Cell Endocrinol 2004, 216: 47–54. 10.1016/j.mce.2003.10.068

    Article  CAS  PubMed  Google Scholar 

  48. Knapen MF, Zusterzeel PL, Peters WH, Steegers EA: Glutathione and glutathione-related enzymes in reproduction. A review. Eur J Obstet Gynecol Reprod Biol 1999, 82: 171–184. 10.1016/S0301-2115(98)00242-5

    Article  CAS  PubMed  Google Scholar 

  49. Osanai M, Chen PS: A comparative study on the arginine degradation cascade for sperm maturation of Bombyx mori and Drosophila melanogaster . Amino Acids 1993, 5: 341–350. 10.1007/BF00806952

    Article  CAS  PubMed  Google Scholar 

  50. Ashburner M, Thompson JN: The laboratory culture of Drosophila . In The Genetics and Biology of Drosophila. Volume 2a. Edited by: Ashburner M, Wright TRF. London: Academic Press; 1978:1–109.

    Google Scholar 

  51. Ashburner M: Drosophila a laboratory manual. New York: Cold Spring Harbor Laboraotry Press; 1989.

    Google Scholar 

  52. Borner GH, Lilley KS, Stevens TJ, Dupree P: Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis. Plant Physiol 2003, 132: 568–577. 10.1104/pp.103.021170

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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We thank Kathryn S Lilley of the Cambridge Centre for Proteomics for LC-MS/MS, Richard J Bingham of the Astbury Centre for Structural Molecular Biology, University of Leeds, for valuable discussion. MJW and CMR were supported by studentships from the Biotechnology and Biological Sciences Research Council, UK.

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Correspondence to R Elwyn Isaac.

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Authors' contributions

MJW, CMR and JNK carried out the proteomic analysis. MJW and MS planned and performed the enzyme activity studies. NA identified the cleavage site for the hydrolysis of sex peptide. REI drafted the manuscript. ADS helped to revise the manuscript and REI and ADS jointly planned the study.

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Walker, M.J., Rylett, C.M., Keen, J.N. et al. Proteomic identification of Drosophila melanogaster male accessory gland proteins, including a pro-cathepsin and a soluble γ-glutamyl transpeptidase. Proteome Sci 4, 9 (2006).

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