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  1. The secretion time course of Bacillus anthracis strain RA3R (pXO1+/pXO2-) during early, mid, and late log phase were investigated under conditions that simulate those encountered in the host. All of the identifie...

    Authors: Alexander Walz, Cesar V Mujer, Joseph P Connolly, Tim Alefantis, Ryan Chafin, Clarissa Dake, Jessica Whittington, Srikanta P Kumar, Akbar S Khan and Vito G DelVecchio
    Citation: Proteome Science 2007 5:11
  2. The adaptive immune response requires waves of T-cell clonal expansion on contact with pathogen and elimination after clearance of the source of antigen. However, lifelong persistent infections with common vir...

    Authors: Dawn J Mazzatti, Graham Pawelec, Robin Longdin, Jonathan R Powell and Rosalyn J Forsey
    Citation: Proteome Science 2007 5:7
  3. The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able to form DNA adducts that ma...

    Authors: Roberta Pastorelli, Federica Saletta, Donatella Carpi, Roberta Campagna, Carlo dell'Osta, Silvia Schiarea, Paolo Vineis, Luisa Airoldi and Giuseppe Matullo
    Citation: Proteome Science 2007 5:6
  4. To identify the pattern of protein expression in the retina from a patient with Leber's Congenital Amaurosis (LCA) secondary to a mutation in the AIPL1 gene. The retina from one eye of a patient with LCA and 7 co...

    Authors: Henrik Vorum, Morten Østergaard, Greg E Rice, Bent Honoré and Toke Bek
    Citation: Proteome Science 2007 5:5
  5. Proteomics continues to play a critical role in post-genomic science as continued advances in mass spectrometry and analytical chemistry support the separation and identification of increasing numbers of pepti...

    Authors: Jennifer A Siepen, Neil Swainston, Andrew R Jones, Sarah R Hart, Henning Hermjakob, Philip Jones and Simon J Hubbard
    Citation: Proteome Science 2007 5:4
  6. Tandem mass spectrometry followed by database search is currently the predominant technology for peptide sequencing in shotgun proteomics experiments. Most methods compare experimentally observed spectra to th...

    Authors: Jian Liu, Alexander W Bell, John JM Bergeron, Corey M Yanofsky, Brian Carrillo, Christian EH Beaudrie and Robert E Kearney
    Citation: Proteome Science 2007 5:3
  7. Urine consists of a complex mixture of peptides and proteins and therefore is an interesting source of biomarkers. Because of its high throughput capacity SELDI-TOF-MS is a proteomics technology frequently use...

    Authors: Han Roelofsen, Gloria Alvarez-Llamas, Marianne Schepers, Karloes Landman and Roel J Vonk
    Citation: Proteome Science 2007 5:2
  8. Ischemia-reperfusion (I/R) causes a dramatic reprogramming of cell metabolism during liver transplantation and can be linked to an alteration of the phosphorylation level of several cellular proteins. Over the...

    Authors: Anouk Emadali, Peter P Metrakos, Fariba Kalantari, Tarek Boutros, Daniel Boismenu and Eric Chevet
    Citation: Proteome Science 2007 5:1
  9. Opiate addiction reflects plastic changes that endurably alter synaptic transmission within relevant neuronal circuits. The biochemical mechanisms of these adaptations remain largely unknown and proteomics-bas...

    Authors: Jérémie Neasta, Sandrine Uttenweiler-Joseph, Karima Chaoui, Bernard Monsarrat, Jean-Claude Meunier and Lionel Moulédous
    Citation: Proteome Science 2006 4:23
  10. The aim of the study was to optimize protocols for finding and identifying serum proteins that are differentially expressed in persons with normal glucose tolerance (NGT) compared to individuals with type 2 di...

    Authors: Tea Sundsten, Michael Eberhardson, Michael Göransson and Peter Bergsten
    Citation: Proteome Science 2006 4:22
  11. The antibody microarray technique is a newly emerging proteomics tool for differential protein expression analyses that uses fluorescent dyes Cy 3 and Cy 5. Environmental factors, such as light exposure, can a...

    Authors: Qiang Gu, Thamil Mani Sivanandam and Caroline Aehyun Kim
    Citation: Proteome Science 2006 4:21
  12. Surface-Enhanced Laser Desorption/Ionization – Time Of Flight (SELDI-TOF) has been proposed as new approach for blood biomarker discovery. However, results obtained so far have been often disappointing as this...

    Authors: Stéphane Roche, Laurent Tiers, Monique Provansal, Marie-Thérèse Piva and Sylvain Lehmann
    Citation: Proteome Science 2006 4:20
  13. High cell density cultivations were performed under identical conditions for two Bacillus megaterium strains (MS941 and WH320), both carrying a heterologous dextransucrase (dsrS) gene under the control of the xyl...

    Authors: Wei Wang, Rajan Hollmann and Wolf-Dieter Deckwer
    Citation: Proteome Science 2006 4:19
  14. The elemental composition of peptides results in formation of distinct, equidistantly spaced clusters across the mass range. The property of peptide mass clustering is used to calibrate peptide mass lists, to ...

    Authors: Witold E Wolski, Malcolm Farrow, Anne-Katrin Emde, Hans Lehrach, Maciej Lalowski and Knut Reinert
    Citation: Proteome Science 2006 4:18
  15. Proteomics may help to detect subtle pollution-related changes, such as responses to mixture pollution at low concentrations, where clear signs of toxicity are absent. The challenges associated with the analys...

    Authors: Tiphaine Monsinjon, Odd Ketil Andersen, François Leboulenger and Thomas Knigge
    Citation: Proteome Science 2006 4:17
  16. Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are no...

    Authors: Ming Jin, Philip T Diaz, Tran Bourgeois, Charis Eng, Clay B Marsh and Haifeng M Wu
    Citation: Proteome Science 2006 4:16
  17. Characterization of protein modification by phosphorylation is one of the major tasks that have to be accomplished in the post-genomic era. Phosphorylation is a key reversible modification occurring mainly on ...

    Authors: Frédéric Delom and Eric Chevet
    Citation: Proteome Science 2006 4:15
  18. We describe the application of an ELISA-based assay (the Peptidomatrix) that can be used to simultaneously identify and quantitate a number of proteins in biological samples. The biological sample (blood compo...

    Authors: Ori Braitbard, Janette Bishara-Shieban, Hava Glickstein, Miriam Kott-Gutkowski, Umberto Pace, Deborah G Rund and Wilfred D Stein
    Citation: Proteome Science 2006 4:14
  19. The extracellular matrix can have a profound effect upon the phenotype of cancer cells. Previous work has shown that growth of bladder cancer cells on a matrix derived from normal basement membrane suppresses ...

    Authors: Robert E Hurst, Kimberly D Kyker, Mikhail G Dozmorov, Nobuaki Takemori, Anil Singh, Hiroyuki Matsumoto, Ricardo Saban, Edna Betgovargez and Michael H Simonian
    Citation: Proteome Science 2006 4:13
  20. We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with ...

    Authors: Ori Braitbard, Hava Glickstein, Janette Bishara-Shieban, Umberto Pace and Wilfred D Stein
    Citation: Proteome Science 2006 4:12
  21. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Previous studies have demonstrated that the biological and pharmacological activities of B. atrox venom alter ...

    Authors: Rafael AP Guércio, Anna Shevchenko, Andrej Shevchenko, Jorge L López-Lozano, Jaime Paba, Marcelo V Sousa and Carlos AO Ricart
    Citation: Proteome Science 2006 4:11
  22. The field of plasmid-based functional proteomics requires the rapid assay of proteins expressed from plasmid libraries. Automation is essential since large sets of mutant open reading frames are being cloned f...

    Authors: Stephen R Hughes, Steven B Riedmuller, Jeffrey A Mertens, Xin-Liang Li, Kenneth M Bischoff, Nasib Qureshi, Michael A Cotta and Philip J Farrelly
    Citation: Proteome Science 2006 4:10
  23. In Drosophila melanogaster, the male seminal fluid contains proteins that are important for reproductive success. Many of these proteins are synthesised by the male accessory glands and are secreted into the acce...

    Authors: Michael J Walker, Caroline M Rylett, Jeff N Keen, Neil Audsley, Mohammed Sajid, Alan D Shirras and R Elwyn Isaac
    Citation: Proteome Science 2006 4:9
  24. Scientific publications should provide sufficient detail in terms of methodology and presented data to enable the community to reproduce the methodology to generate similar data and arrive at the same conclusi...

    Authors: Martin Latterich
    Citation: Proteome Science 2006 4:8
  25. Cerebrospinal fluid (CSF) potentially carries an archive of peptides and small proteins relevant to pathological processes in the central nervous system (CNS) and surrounding brain tissue. Proteomics is especi...

    Authors: Nelson Guerreiro, Baltazar Gomez-Mancilla and Stéphane Charmont
    Citation: Proteome Science 2006 4:7
  26. Halobacterium sp. NRC-1 is an extremely halophilic archaeon and has adapted to optimal growth under conditions of extremely high salinity. Its proteome is highly acidic with a median pI of 4.9, a unique character...

    Authors: Hem D Shukla
    Citation: Proteome Science 2006 4:6
  27. In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerpri...

    Authors: Gabriele D'Andrea, Anna R Lizzi, Sara Venditti, Laura Di Francesco, Alessandra Giorgi, Giuseppina Mignogna, Arduino Oratore and Argante Bozzi
    Citation: Proteome Science 2006 4:4
  28. Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction,...

    Authors: Tsz-Kwong Man, Yiting Li, Tu Anh Dang, Jianhe Shen, Laszlo Perlaky and Ching C Lau
    Citation: Proteome Science 2006 4:3
  29. This study presents the development and application of protein lysate microarray (LMA) technology for verification of presence and quantification of human tissue samples for protein biomarkers. Sub-picogram ra...

    Authors: Anitha Ramaswamy, E Lin, Iou Chen, Rahul Mitra, Joel Morrisett, Kevin Coombes, Zhenlin Ju and Mini Kapoor
    Citation: Proteome Science 2005 3:9
  30. The observed molecular weight of a protein on a 1D polyacrylamide gel can provide meaningful insight into its biological function. Differences between a protein's observed molecular weight and that predicted b...

    Authors: Q Rushdy Ahmad, Dat H Nguyen, Mark A Wingerd, George M Church and Martin A Steffen
    Citation: Proteome Science 2005 3:6
  31. The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocol...

    Authors: Matthew A Churchward, R Hussain Butt, John C Lang, Kimberly K Hsu and Jens R Coorssen
    Citation: Proteome Science 2005 3:5
  32. A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivatio...

    Authors: Wei Wang, Rajan Hollmann, Tobias Fürch, Manfred Nimtz, Marco Malten, Dieter Jahn and Wolf-Dieter Deckwer
    Citation: Proteome Science 2005 3:4
  33. Neuroglobin is a hexacoordinated member of the globin family of proteins. It is predominantly localized to various brain regions and retina where it may play a role in protection against ischemia and nitric ox...

    Authors: Begona Casado, Lewis K Pannell, Gail Whalen, Daniel J Clauw and James N Baraniuk
    Citation: Proteome Science 2005 3:2
  34. The structural integrity of recombinant proteins is of critical importance to their application as clinical treatments. Recombinant growth hormone preparations have been examined by several methodologies. In t...

    Authors: Felix Hepner, Edina Cszasar, Elisabeth Roitinger and Gert Lubec
    Citation: Proteome Science 2005 3:1
  35. Quantitative proteomics is an emerging field that encompasses multiplexed measurement of many known proteins in groups of experimental samples in order to identify differences between groups. Antibody arrays a...

    Authors: LT Perlee, J Christiansen, R Dondero, B Grimwade, S Lejnine, M Mullenix, W Shao, M Sorette, VT Tchernev, DD Patel and SF Kingsmore
    Citation: Proteome Science 2004 2:9
  36. Chaperones (CH) play an important role in tumor biology but no systematic work on expressional patterns has been reported so far. The aim of the study was therefore to present an analytical method for the conc...

    Authors: Jae-Kyung Myung, Leila Afjehi-Sadat, Maureen Felizardo-Cabatic, Irene Slavc and Gert Lubec
    Citation: Proteome Science 2004 2:8
  37. The aim of this study was firstly, to improve and validate a cerebrospinal fluid (CSF) prefractionation method followed by two-dimensional electrophoresis (2-DE) and secondly, using this strategy to investigat...

    Authors: Sara Folkesson Hansson, Maja Puchades, Kaj Blennow, Magnus Sjögren and Pia Davidsson
    Citation: Proteome Science 2004 2:7
  38. Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this ap...

    Authors: Guennadi A Khoudoli, Iain M Porter, J Julian Blow and Jason R Swedlow
    Citation: Proteome Science 2004 2:6
  39. Structural genomics (SG) projects aim to determine thousands of protein structures by the development of high-throughput techniques for all steps of the experimental structure determination pipeline. Crucial t...

    Authors: Stéphane Raymond, Nicholas O'Toole and Miroslaw Cygler
    Citation: Proteome Science 2004 2:4

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